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High voltage electric discharge extraction of chlorogenic acid from tobacco industrial waste

The chlorogenic acid (3-O-Caffeoylquinic acid) is a member of a group of caffeoylquinic acids, commercially delivered from Lonicera japonica Thunb and Eucommia ulmoides Oliver plants. It can be naturally found in green coffee beans, tea leaves and tobacco. Recently, the chlorogenic acid and its isomers have received increasing attention due to its multiple pharmacological effects and biological activities such as antioxidant, antibacterial, antiobesity, anti-inflammatory, antitumor, antihypertensive, and gastrointestinal tract-protective effects. This study aimed to evaluate the potential of high voltage electrical discharge in the extraction of chlorogenic acid from tobacco industrial waste, as an alternative source of this important compound. Extraction was conducted using high voltage electric discharge procedure under various process conditions: solvent/solid ratio (from 300 to 700 mL/g), treatment time (from 15 to 45 min) and frequency (from 40 to 100 Hz), to study the effect of these conditions on the concentration of chlorogenic acid. The optimal extraction conditions are defined as follow, solvent/solid ratio: 695.24 mL/g, frequency: 47.16 Hz and extraction time: 15.06 min. The present study clarified that tobacco waste is a rich source of chlorogenic acid that could be recovered and used for many purposes as a food additive or nutraceutical.

chlorogenic acid ; high voltage electric discharge ; extraction ; tobacco waste

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