engleski

Melittin stability in honey bee venom under different storage conditions measured with RP- HPLC-PDA method

The aim of this study was to determine performance characteristics of reversed-phase high performance liquid chromatographic method with photo-diode array detector (RP-HPLC-PDA) for melittin content determination, and to evaluate the melittin content in honey bee venom during 6 months of storage at room temperature and in the freezer. Based on the obtained results of the method performance characteristics(linearity, precision, trueness, LOD and LOQ), the used RP-HPLC-PDA method is fit for purpose. Linearity was confirmed with correlation coefficient of 0.999 while relative standard deviation (RSD) of sample preparation repeatability was 2.45 %. Limits of detection and quantification were 0.62 μg/mL and 1.88 μg/mL, respectively. Statistical analysis showed that the storage temperature had no effect on the melittin content during 6 months of storage. The results of this study can be of great interest to the beekeepers because it show that venom quality is maintained with reduced cost for storing and manipulation.

honey bee venom ; melittin ; RP-HPLC-PDA method ; stability ; storage

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