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In vitro gastrointestinal stability and bioaccessibility of glucosinolates from selected plants of the order Brassicales

Glucosinolates, compounds present in Brassica vegetables, provide several benefits to human health, such as anticancer, antimicrobial, antihyperglycemic, anti-inflammatory, and etc. Therefore, it is extremely important to evaluate the rate of stability and bioaccessibility of glucosinolates after digestive process. Static in vitro digestion methods are the simplest methods for simulation of in vivo digestion model. The concept of a compound bioaccessibility has been defined as the fraction released from the food matrix in the gastrointestinal tract that becomes available for absorption. Therefore, the aims of this research were to determine glucosinolates in the plant seeds of different mustards and in Tropaeolum majus L. that belong to the order Brassicales, and their stability and bioaccessibility after two simulated digestion methods - in vitro method based on the use of commercial enzymes from the stomach and small intestine, and in vitro method with human digestive juices from the stomach and small intestine. Identification of glucosinolates, their in vitro gastrointestinal stability and bioaccessibility were determined using high pressure liquid chromatography - tandem mass spectrometry (HPLC-MS/MS). Glucosinolates showed higher stability after gastric digestion phase in comparison with intestinal phase.

order Brassicales, glucosinolates, in vitro digestion method, gastrointestinal stability and bioaccessibility, HPLC-MS/MS

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