Sex differences in oxidative stress level and antioxidative enzymes expression and activity in obese pre-diabetic elderly rats treated with metformin or liraglutide (CROSBI ID 298267)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Matić, Anita ; Vuković, Rosemary ; Heffer, Marija ; Balog, Marta ; Ivić, Vedrana ; Gaspar, Robert ; Ducza, Eszter ; Szucs, Kalman F ; Seres, Adrienn ; Vari, Sandor G ; Drenjančević, Ines
engleski
Sex differences in oxidative stress level and antioxidative enzymes expression and activity in obese pre-diabetic elderly rats treated with metformin or liraglutide
Aim: To determine the effects of metformin or liraglutide on oxidative stress level and antioxidative enzymes gene expression and activity in the blood and vessels of pre-diabetic obese elderly Sprague-Dawley (SD) rats of both sexes. Methods: Male and female SD rats were assigned to the following groups: a) control group (fed with standard rodent chow) ; b) high- fat and high- carbohydrate diet (HSHFD) group fed with HSHFD from 20-65 weeks of age ; c) HSHFD+metformin treatment (50 mg/kg/d s.c.) ; and d)HSHFD+liraglutide treatment (0.3 mg/kg/d s.c). Oxidative stress parameters (ferric reducing ability of plasma and thiobarbituric acid reactive substances) and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase(GPx) activity and gene expression were determined from serum, aortas, and surface brain blood vessels (BBV). Results: HSHFD increased body weight in both sexes compared with the control group, while liraglutide prevented this increase. Blood glucose level did not change. The liraglutide group had a significantly increased antioxidative capacity compared with the HSHFD group in both sexes. The changes in antioxidative enzymes’ activities in plasma were more pronounced in male groups. The changes in gene expression of antioxidative enzymes were more prominent in microvessels and may be attributed to weight gain prevention. Conclusions: Obesity and antidiabetic drugs caused sexrelated differences in the level of antioxidative parameters. Liraglutide exhibited stronger antioxidative effects than metformin. These results indicate that weight gain due to HSHFD is crucial for developing oxidative stress and for inhibiting antioxidative protective mechanisms.
antioxidative enzymes ; liraglutide ; metformin ; oxidative stress ; pre-diabetic obese elderly Sprague-Dawley rats
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Podaci o izdanju
62 (3)
2021.
215-226
objavljeno
0353-9504
1332-8166
10.3325/cmj.2021.62.215
Povezanost rada
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)