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Pregled bibliografske jedinice broj: 1141244

Zn(II) and free base N-methylated tripyridylporphyrins: impact of solubility and light excitation wavelength on PDT effect


Mušković, Martina; Džeba, Iva; Basarić, Nikola; Lončarić, Martin; Mihaljević, Branka; Ratkaj, Ivana; Malatesti, Nela
Zn(II) and free base N-methylated tripyridylporphyrins: impact of solubility and light excitation wavelength on PDT effect // 19th Congress of the European Society for Photobiology
Salzburg, Austrija, 2021. str. 254-254 (poster, međunarodna recenzija, sažetak, znanstveni)


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Naslov
Zn(II) and free base N-methylated tripyridylporphyrins: impact of solubility and light excitation wavelength on PDT effect

Autori
Mušković, Martina ; Džeba, Iva ; Basarić, Nikola ; Lončarić, Martin ; Mihaljević, Branka ; Ratkaj, Ivana ; Malatesti, Nela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
19th Congress of the European Society for Photobiology

Mjesto i datum
Salzburg, Austrija, 30.08-03.09.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Amphiphilic porphyrins ; Zn(II) chelation ; Photodynamic therapy ; Melanoma

Sažetak
Amphiphilic cationic porphyrins have shown high potential in use as photosensitizers (PSs) for photodynamic therapy (PDT). Our research group has shown that compounds conjugated with long chain (C18) have an exceptional PDT effect compared to hydrophilic analogues on different tested cell lines. Unfortunately, highly effective amphiphilic compounds showed reduced selectivity to tumour cells, relatively high dark toxicity, and their aggregation was observed in high concentration. It must be kept in mind that the optimal ratio of hydrophilic and hydrophobic moieties is necessary to facilitate passage through the membrane bilayer, while maintaining good solubility in water and avoiding formation of aggregates that reduce the PDT effect. Chelation with Zn(II) alters the properties of free base porphyrins ; it changes lipophilicity of the compounds and may increase the lifetime of the excited triplet state (3PS*). The change also occurs in the absorbance spectra, as expected the number of Q bands reduces from four to two and there is a negligible absorption above 610 nm for these compounds to be efficiently activated with red. Therefore, we suggest photoactivation using LED based source of orange light, which better corresponds to the optical properties of Zn(II) porphyrins while maintaining good penetration through the tissue.In this work, we will present the synthesis of two groups of N- methylated tripyridylporphyrins, free base and chelated with Zn(II), both conjugated to alkyl chains of different length. Their photophysical and photochemical properties studied in different solvents, by laser flash photolysis (LFP) and timeresolved fluorescence spectroscopy (TRF) will be presented. The impact of small changes in chain length will also be evaluated in vitro, using MTT assay to detect cytotoxicity and measuring cellular uptake by using fluorescence microscopy on melanoma cells (MeWo) and human foreskin fibroblasts (HFF). To study the effect of the irradiation wavelength, the cytotoxicity of the compounds will be evaluated using red light (645 nm ; fluence rate 2.0 mW / cm2) and orange light (605 nm ; fluence rate 1.5 mW / cm2).

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Biologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA


Projekti:
NadSve-Sveučilište u Rijeci-uniri-prirod-18-173 - Priprema lipidnih konjugata piridilporfirina, njihova karakterizacija i fotodinamička aktivnost (Malatesti, Nela, NadSve - UNIRI PROJEKTI) ( POIROT)
EK-EFRR-RC.2.2.06-0001 - Razvoj istraživačke infrastrukture na kampusu Sveučilišta u Rijeci (RISK) (Ožanić, Nevenka, EK - Operativni program Regionalna konkurentnost) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb,
Sveučilište u Rijeci - Odjel za biotehnologiju


Citiraj ovu publikaciju

Mušković, Martina; Džeba, Iva; Basarić, Nikola; Lončarić, Martin; Mihaljević, Branka; Ratkaj, Ivana; Malatesti, Nela
Zn(II) and free base N-methylated tripyridylporphyrins: impact of solubility and light excitation wavelength on PDT effect // 19th Congress of the European Society for Photobiology
Salzburg, Austrija, 2021. str. 254-254 (poster, međunarodna recenzija, sažetak, znanstveni)
Mušković, M., Džeba, I., Basarić, N., Lončarić, M., Mihaljević, B., Ratkaj, I. & Malatesti, N. (2021) Zn(II) and free base N-methylated tripyridylporphyrins: impact of solubility and light excitation wavelength on PDT effect. U: 19th Congress of the European Society for Photobiology.
@article{article, year = {2021}, pages = {254-254}, keywords = {Amphiphilic porphyrins, Zn(II) chelation, Photodynamic therapy, Melanoma}, title = {Zn(II) and free base N-methylated tripyridylporphyrins: impact of solubility and light excitation wavelength on PDT effect}, keyword = {Amphiphilic porphyrins, Zn(II) chelation, Photodynamic therapy, Melanoma}, publisherplace = {Salzburg, Austrija} }
@article{article, year = {2021}, pages = {254-254}, keywords = {Amphiphilic porphyrins, Zn(II) chelation, Photodynamic therapy, Melanoma}, title = {Zn(II) and free base N-methylated tripyridylporphyrins: impact of solubility and light excitation wavelength on PDT effect}, keyword = {Amphiphilic porphyrins, Zn(II) chelation, Photodynamic therapy, Melanoma}, publisherplace = {Salzburg, Austrija} }




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