engleski

Surface method approach in characterization of plasma membrane vesicles derived from algal cells

Microalgae are important biological resources that have a wide range of biotechnological applications, especially in terms of pigment and metabolite isolation, while the potential of the plasma membrane vesicles [1] has not been investigated extensively. This study aims to prepare and characterize algal plasma membrane- derived from algal cells by two complementary surface methods. Polarography and chronoamperometry at the dropping mercury electrode enable to examine adhesion behavior of plasma membrane vesicles in respect of applied potential. Results show that fluid vesicles adhere at the wide potential range from -180 mV to – 900 mV, which corresponds to the surface charge density from +21 µC cm-2 to -13 µC cm-2 [2]. The distribution of signal amplitudes of plasma membrane vesicles reflects vesicle size, while signal frequency is related to vesicle concentration in suspension. Atomic force microscopy imaging provides the structural characterization of adhered plasma membrane vesicles on mica surface in air. Results show that the plasma membrane vesicles are in the size range of 5-30 μm, the membranes are spherical, nearly empty, with a membrane height of about 20 nm. In the future expanded knowledge of this unique membrane system could contribute to the design of marine bioinspired materials for the development of drug delivery systems. [1] N. Ivošević DeNardis, G. Pletikapić, R. Frkanec, L. Horvat, P. T. Vernier, Bioelectrochemistry 134 (2020) 1-8. [2] N. Novosel, N. Ivošević DeNardis, Electroanalysis 33 (2021) 1-9.

plasma membrane vesicles ; microalgae ; AFM

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