engleski

A pilot study of fresh wheat grass juice intestine absorption by Caco -2 model in vitro: Fe, Zn and Mg abs orption for a vivid cel

Wheatgrass – WG (Triticum aestivum) known as “living food” possess a variety of biological activities. It is rich source of proteins, vitamins, antioxidants, and minerals. Wheatgrass is a common health supplement, consumed in the form of fresh juice, powder or tablet to boost up the health and vitality. Since most of supplements are orally ingested, big question is active concentration of components absorbed cross the intestinal barrier. Caco-2 cell system is intestinal in vitro model which enables evaluation of compound transport mechanism and ability to pass through enterocyte membrane. Permeability values estimated with Caco-2 model correlate well with human in vivo absorption. The aim of this study is to establish cultivation conditions for Caco-2 model to determinate active concentrations of three minerals (Mg, Zn, Fe) indispensable for cells and enzyme activity. Five variety of WG were germinated over 12 days in controlled conditions (tem. 20°C, day and night light (10/14 h). Samples of WG were centrifuged (3000 rpm/10 min/RT) and applied to cells diluted (1:4) in HBSS/MES buffer. As a standard, commercial WG powder was applied. Samples were collected every 20 minutes (0–100 min) in duplicate and stored at -80°C before analysis.Concentration in time dependent manner were estimated by AAS. Caco-2 model was confirmed by measurement of TEER value for each well on the 19th day of cultivation. Concentration range for Zn, Fe and Mg were respectively as follows: 0 min (117.16 ; 826.54 ; 46.3 x103 µg/L), 20 min (93.85 ; 387.26 ; 23.13 x103 µg/L), 40 min (44.45 ; 66.92 ; 6.85 x103 µg/L), 60 min (29.45 ; 104.49 ; 6.54 x103 µg/L), 80 min (15.23 ; 54.24 ; 2.79 x103 µg/L), 100 min (6.67 ; 46.21 ; 1.75 x103 µg/L). Results indicate passive model of transport for analyzed minerals with well notable tendency of reduced rate of absorption through the time. The highest rate of absorption, as anticipated, was in the first 20 minutes pointing to medium speed absorptive compounds

Caco-2 model ; intestinal absorption ; Wheatgrass ; TEER

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