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Pregled bibliografske jedinice broj: 1138309

Altered Root Growth, Auxin Metabolism and Distribution in Arabidopsis thaliana Exposed to Salt and Osmotic Stress


Smolko, Ana; Bauer, Nataša; Pavlović, Iva; Penčik, Aleš; Novák, Ondrej; Salopek-Sondi, Branka
Altered Root Growth, Auxin Metabolism and Distribution in Arabidopsis thaliana Exposed to Salt and Osmotic Stress // International journal of molecular sciences, 22 (2021), 7993, 21 doi:10.3390/ ijms22157993 (međunarodna recenzija, članak, znanstveni)


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Naslov
Altered Root Growth, Auxin Metabolism and Distribution in Arabidopsis thaliana Exposed to Salt and Osmotic Stress

Autori
Smolko, Ana ; Bauer, Nataša ; Pavlović, Iva ; Penčik, Aleš ; Novák, Ondrej ; Salopek-Sondi, Branka

Izvornik
International journal of molecular sciences (1422-0067) 22 (2021); 7993, 21

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
abiotic stress ; Arabidopsis thaliana ; auxin distribution ; auxin metabolome ; auxin transcriptome ; root growth

Sažetak
Salt and osmotic stress are the main abiotic stress factors affecting plant root growth and architecture. We investigated the effect of salt (100 mM NaCl) and osmotic (200 mM mannitol) stress on the auxin metabolome by UHPLC-MS/MS, auxin distribution by confocal microscopy, and transcript levels of selected genes by qRT-PCR in Arabidopsis thaliana ecotype Columbia-0 (Col-0) and DR5rev::GFP (DR5) line. During long-term stress (13 days), a stability of the auxin metabolome and a tendency to increase indole-3- acetic acid (IAA) were observed, especially during salt stress. Short-term stress (3 h) caused significant changes in the auxin metabolome, especially NaCl treatment resulted in a significant reduction of IAA. The data derived from auxin profiling were consistent with gene expressions showing the most striking changes in the transcripts of YUC, GH3, and UGT transcripts, suggesting disruption of auxin biosynthesis, but especially in the processes of amide and ester conjugation. These data were consistent with the auxin distribution observed in the DR5 line. Moreover, NaCl treatment caused a redistribution of auxin signals from the quiescent center and the inner layers of the root cap to the epidermal and cortical cells of the root elongation zone. The distribution of PIN proteins was also disrupted by salt stress ; in particular, PIN2 was suppressed, even after 5 min of treatment. Based on our results, the DR5 line was more sensitive to the applied stresses than Col-0, although both lines showed similar trends in root morphology, as well as transcriptome and metabolome parameters under stress conditions.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
EK-EFRR-KK.05.1.1.02.0005 - Agrobioraznolikost - osnova za prilagodbu i ublažavanje posljedica klimatskih promjena u poljoprivredi (Goreta Ban, Smiljana; Salopek Sondi, Branka; Bauer, Nataša; Jasprica, Nenad; Kereša, Snježana, EK - KK.05.1.1.02) ( POIROT)
HRZZ-IP-2014-09-4359 - Fitohormoni u abiotskom stresu kupusnjača: mehanizam tolerancije i primjena (PhytoBraCro) (Salopek-Sondi, Branka, HRZZ - 2014-09) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Iva Pavlović (autor)

Avatar Url Nataša Bauer (autor)

Avatar Url Branka Salopek-Sondi (autor)

Avatar Url Ana Smolko (autor)

Citiraj ovu publikaciju:

Smolko, Ana; Bauer, Nataša; Pavlović, Iva; Penčik, Aleš; Novák, Ondrej; Salopek-Sondi, Branka
Altered Root Growth, Auxin Metabolism and Distribution in Arabidopsis thaliana Exposed to Salt and Osmotic Stress // International journal of molecular sciences, 22 (2021), 7993, 21 doi:10.3390/ ijms22157993 (međunarodna recenzija, članak, znanstveni)
Smolko, A., Bauer, N., Pavlović, I., Penčik, A., Novák, O. & Salopek-Sondi, B. (2021) Altered Root Growth, Auxin Metabolism and Distribution in Arabidopsis thaliana Exposed to Salt and Osmotic Stress. International journal of molecular sciences, 22, 7993, 21 doi:10.3390/ ijms22157993.
@article{article, author = {Smolko, Ana and Bauer, Nata\v{s}a and Pavlovi\'{c}, Iva and Pen\v{c}ik, Ale\v{s} and Nov\'{a}k, Ondrej and Salopek-Sondi, Branka}, year = {2021}, pages = {21}, DOI = {10.3390/ ijms22157993}, chapter = {7993}, keywords = {abiotic stress, Arabidopsis thaliana, auxin distribution, auxin metabolome, auxin transcriptome, root growth}, journal = {International journal of molecular sciences}, doi = {10.3390/ ijms22157993}, volume = {22}, issn = {1422-0067}, title = {Altered Root Growth, Auxin Metabolism and Distribution in Arabidopsis thaliana Exposed to Salt and Osmotic Stress}, keyword = {abiotic stress, Arabidopsis thaliana, auxin distribution, auxin metabolome, auxin transcriptome, root growth}, chapternumber = {7993} }
@article{article, author = {Smolko, Ana and Bauer, Nata\v{s}a and Pavlovi\'{c}, Iva and Pen\v{c}ik, Ale\v{s} and Nov\'{a}k, Ondrej and Salopek-Sondi, Branka}, year = {2021}, pages = {21}, DOI = {10.3390/ ijms22157993}, chapter = {7993}, keywords = {abiotic stress, Arabidopsis thaliana, auxin distribution, auxin metabolome, auxin transcriptome, root growth}, journal = {International journal of molecular sciences}, doi = {10.3390/ ijms22157993}, volume = {22}, issn = {1422-0067}, title = {Altered Root Growth, Auxin Metabolism and Distribution in Arabidopsis thaliana Exposed to Salt and Osmotic Stress}, keyword = {abiotic stress, Arabidopsis thaliana, auxin distribution, auxin metabolome, auxin transcriptome, root growth}, chapternumber = {7993} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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