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Pregled bibliografske jedinice broj: 1138192

Expression Pattern of 5-HT (Serotonin) Receptors during Normal Development of the Human Spinal Cord and Ganglia and in Fetus with Cervical Spina Bifida


Punda, Hrvoje; Mardešić, Snježana; Filipović, Natalija; Kosović, Ivona; Benzon, Benjamin; Ogorevc, Marin; Bočina, Ivana; Kolić, Krešimir; Vukojević, Katarina; Saraga- Babić, Mirna
Expression Pattern of 5-HT (Serotonin) Receptors during Normal Development of the Human Spinal Cord and Ganglia and in Fetus with Cervical Spina Bifida // International journal of molecular sciences, 22 (2021), 14; 7320, 20 doi:10.3390/ijms22147320 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1138192 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Expression Pattern of 5-HT (Serotonin) Receptors during Normal Development of the Human Spinal Cord and Ganglia and in Fetus with Cervical Spina Bifida

Autori
Punda, Hrvoje ; Mardešić, Snježana ; Filipović, Natalija ; Kosović, Ivona ; Benzon, Benjamin ; Ogorevc, Marin ; Bočina, Ivana ; Kolić, Krešimir ; Vukojević, Katarina ; Saraga- Babić, Mirna

Izvornik
International journal of molecular sciences (1422-0067) 22 (2021), 14; 7320, 20

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
spinal cord ; ganglia ; human development ; cervical spina bifida ; serotonin

Sažetak
The expression of 5-HT (serotonin) receptors (sr) was analyzed in the spinal cord and ganglia of 15 human conceptuses (5-10- weeks), and in the 9-week fetus with spina bifida. We used immunohistochemical method to detect sr-positive, apoptotic (caspase-3) and proliferating (Ki-67) cells, double immunofluorescence for co-localization with protein gene peptide (pgp) 9.5 and GFAP, as well as semiquantification and statistical measurements. Following the neurulation process, moderate (sr1 and sr2) and mild (sr3) expression characterized neuroblasts in the spinal cord and ganglia. During further development, sr1 expression gradually increased in the motoneurons, autonomic and sensory neurons, while sr2 and sr3 increased strongly in floor and roof plates. In the ganglia, sr3 expression increased during limited developmental period, while sr1 and sr2 increased throughout the investigated period. Co-expression of sr/pgp 9.5 characterized developing neurons, while sr/GFAP co- localized in the roof plate. In the spinal cord and ganglia of malformed fetus, weaker sr1 and sr2 and stronger sr3 expression accompanied morphological abnormalities. Anomalous roof plate morphology showed an excess of apoptotic and proliferating cells and increased sr3 expression. Our results indicate a human-species specific sr expression pattern, and the importance of sr1 in neuronal differentiation, and sr2 and sr3 in the control of the roof plate morphogenesis in normal and disturbed development.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
MZOS-216-2160528-0507 - Genski izražaj u ranom razvoju čovjeka (Saraga-Babić, Mirna, MZOS ) ( POIROT)
HRZZ-IP-2016-06-2575 - Karakterizacija kandidat gena za kongenitalne anomalije bubrega i urotrakta (CAKUT) tijekom razvoja u miša i čovjeka (CAKUT) (Vukojević, Katarina, HRZZ - 2016-06) ( POIROT)

Ustanove:
KBC Split,
Prirodoslovno-matematički fakultet, Split,
Medicinski fakultet, Split

Poveznice na cjeloviti tekst rada:

doi www.mdpi.com

Citiraj ovu publikaciju:

Punda, Hrvoje; Mardešić, Snježana; Filipović, Natalija; Kosović, Ivona; Benzon, Benjamin; Ogorevc, Marin; Bočina, Ivana; Kolić, Krešimir; Vukojević, Katarina; Saraga- Babić, Mirna
Expression Pattern of 5-HT (Serotonin) Receptors during Normal Development of the Human Spinal Cord and Ganglia and in Fetus with Cervical Spina Bifida // International journal of molecular sciences, 22 (2021), 14; 7320, 20 doi:10.3390/ijms22147320 (međunarodna recenzija, članak, znanstveni)
Punda, H., Mardešić, S., Filipović, N., Kosović, I., Benzon, B., Ogorevc, M., Bočina, I., Kolić, K., Vukojević, K. & Saraga- Babić, M. (2021) Expression Pattern of 5-HT (Serotonin) Receptors during Normal Development of the Human Spinal Cord and Ganglia and in Fetus with Cervical Spina Bifida. International journal of molecular sciences, 22 (14), 7320, 20 doi:10.3390/ijms22147320.
@article{article, author = {Punda, Hrvoje and Marde\v{s}i\'{c}, Snje\v{z}ana and Filipovi\'{c}, Natalija and Kosovi\'{c}, Ivona and Benzon, Benjamin and Ogorevc, Marin and Bo\v{c}ina, Ivana and Koli\'{c}, Kre\v{s}imir and Vukojevi\'{c}, Katarina and Saraga- Babi\'{c}, Mirna}, year = {2021}, pages = {20}, DOI = {10.3390/ijms22147320}, chapter = {7320}, keywords = {spinal cord, ganglia, human development, cervical spina bifida, serotonin}, journal = {International journal of molecular sciences}, doi = {10.3390/ijms22147320}, volume = {22}, number = {14}, issn = {1422-0067}, title = {Expression Pattern of 5-HT (Serotonin) Receptors during Normal Development of the Human Spinal Cord and Ganglia and in Fetus with Cervical Spina Bifida}, keyword = {spinal cord, ganglia, human development, cervical spina bifida, serotonin}, chapternumber = {7320} }
@article{article, author = {Punda, Hrvoje and Marde\v{s}i\'{c}, Snje\v{z}ana and Filipovi\'{c}, Natalija and Kosovi\'{c}, Ivona and Benzon, Benjamin and Ogorevc, Marin and Bo\v{c}ina, Ivana and Koli\'{c}, Kre\v{s}imir and Vukojevi\'{c}, Katarina and Saraga- Babi\'{c}, Mirna}, year = {2021}, pages = {20}, DOI = {10.3390/ijms22147320}, chapter = {7320}, keywords = {spinal cord, ganglia, human development, cervical spina bifida, serotonin}, journal = {International journal of molecular sciences}, doi = {10.3390/ijms22147320}, volume = {22}, number = {14}, issn = {1422-0067}, title = {Expression Pattern of 5-HT (Serotonin) Receptors during Normal Development of the Human Spinal Cord and Ganglia and in Fetus with Cervical Spina Bifida}, keyword = {spinal cord, ganglia, human development, cervical spina bifida, serotonin}, chapternumber = {7320} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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