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Pregled bibliografske jedinice broj: 1137264

High-throughput rat immunoglobulin G N-glycosylation profiling revealed subclass-specific changes associated with chronic stress


Habazin, Siniša; Mlinarević, Dražen; Balog, Marta; Bardak, Ana; Gaspar, Robert; Szűcs, Kálmán Ferenc; Vari, Sandor G.; Vučković, Frano; Lauc, Gordan; Novokmet, Mislav; Heffer, Marija
High-throughput rat immunoglobulin G N-glycosylation profiling revealed subclass-specific changes associated with chronic stress // Journal of Proteomics, 245 (2021), 104293, 9 doi:10.1016/j.jprot.2021.104293 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1137264 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
High-throughput rat immunoglobulin G N-glycosylation profiling revealed subclass-specific changes associated with chronic stress

Autori
Habazin, Siniša ; Mlinarević, Dražen ; Balog, Marta ; Bardak, Ana ; Gaspar, Robert ; Szűcs, Kálmán Ferenc ; Vari, Sandor G. ; Vučković, Frano ; Lauc, Gordan ; Novokmet, Mislav ; Heffer, Marija

Izvornik
Journal of Proteomics (1874-3919) 245 (2021); 104293, 9

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
N-glycans ; Glycoproteomics ; Chronic stress ; HPA axis

Sažetak
Immunoglobulin G (IgG) glycosylation corresponds well with immune system changes, so it can potentially be used as a biomarker for the consequences of chronic stress such as low-grade inflammation and enhanced immunosenescence in older animals. Here we present a high-throughput glycoproteomic workflow, including IgG enrichment, HILIC glycopeptide purification, and nano-LC-MS analysis of tryptic glycopeptides applied for the analysis of rat IgG. A cohort of 80 animals was exposed to seven stressors in a customized chronic stress protocol with blood and tissue sampling in three timepoints. Young female rats experienced an increase in agalactosylated glycoforms on IgG2a and IgG2c accompanied by a decrease in monogalactosylation. Among old females, increased galactosylation was observed in the IgG2b subclass, pointing to an anti-inflammatory activity of IgG. Additionally, IgG Fc N-glycosylation patterns in Sprague Dawley rats were analyzed, quantified, and reported for the first time. Our findings emphasize age-, sex- and subclass-dependent differences in IgG glycosylation related to chronic stress exposure, confirming the relevance of newly developed methods for further research in glycobiology of rodent immune response.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Projekti:
EK-KF-KK.01.1.1.01.0010 - Znanstveni centar izvrsnosti za personaliziranu brigu o zdravlju (ZCIPersonHealth) (Secenji, Aleksandar, EK - KK.01.1.1.01) ( POIROT)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Klinički bolnički centar Osijek,
Medicinski fakultet, Osijek,
GENOS d.o.o.

Profili:

Avatar Url Gordan Lauc (autor)

Avatar Url Marta Balog (autor)

Avatar Url Mislav Novokmet (autor)

Avatar Url Marija Heffer (autor)

Avatar Url Frano Vučković (autor)

Avatar Url Siniša Habazin (autor)

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com

Citiraj ovu publikaciju:

Habazin, Siniša; Mlinarević, Dražen; Balog, Marta; Bardak, Ana; Gaspar, Robert; Szűcs, Kálmán Ferenc; Vari, Sandor G.; Vučković, Frano; Lauc, Gordan; Novokmet, Mislav; Heffer, Marija
High-throughput rat immunoglobulin G N-glycosylation profiling revealed subclass-specific changes associated with chronic stress // Journal of Proteomics, 245 (2021), 104293, 9 doi:10.1016/j.jprot.2021.104293 (međunarodna recenzija, članak, znanstveni)
Habazin, S., Mlinarević, D., Balog, M., Bardak, A., Gaspar, R., Szűcs, K., Vari, S., Vučković, F., Lauc, G., Novokmet, M. & Heffer, M. (2021) High-throughput rat immunoglobulin G N-glycosylation profiling revealed subclass-specific changes associated with chronic stress. Journal of Proteomics, 245, 104293, 9 doi:10.1016/j.jprot.2021.104293.
@article{article, author = {Habazin, Sini\v{s}a and Mlinarevi\'{c}, Dra\v{z}en and Balog, Marta and Bardak, Ana and Gaspar, Robert and Sz\H{u}cs, K\'{a}lm\'{a}n Ferenc and Vari, Sandor G. and Vu\v{c}kovi\'{c}, Frano and Lauc, Gordan and Novokmet, Mislav and Heffer, Marija}, year = {2021}, pages = {9}, DOI = {10.1016/j.jprot.2021.104293}, chapter = {104293}, keywords = {N-glycans, Glycoproteomics, Chronic stress, HPA axis}, journal = {Journal of Proteomics}, doi = {10.1016/j.jprot.2021.104293}, volume = {245}, issn = {1874-3919}, title = {High-throughput rat immunoglobulin G N-glycosylation profiling revealed subclass-specific changes associated with chronic stress}, keyword = {N-glycans, Glycoproteomics, Chronic stress, HPA axis}, chapternumber = {104293} }
@article{article, author = {Habazin, Sini\v{s}a and Mlinarevi\'{c}, Dra\v{z}en and Balog, Marta and Bardak, Ana and Gaspar, Robert and Sz\H{u}cs, K\'{a}lm\'{a}n Ferenc and Vari, Sandor G. and Vu\v{c}kovi\'{c}, Frano and Lauc, Gordan and Novokmet, Mislav and Heffer, Marija}, year = {2021}, pages = {9}, DOI = {10.1016/j.jprot.2021.104293}, chapter = {104293}, keywords = {N-glycans, Glycoproteomics, Chronic stress, HPA axis}, journal = {Journal of Proteomics}, doi = {10.1016/j.jprot.2021.104293}, volume = {245}, issn = {1874-3919}, title = {High-throughput rat immunoglobulin G N-glycosylation profiling revealed subclass-specific changes associated with chronic stress}, keyword = {N-glycans, Glycoproteomics, Chronic stress, HPA axis}, chapternumber = {104293} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





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