engleski

Impact of selected pesticides on cholinesterase efficiency

Residual presence of pesticides in a variety of fruits, vegetables and agricultural crops nowadays can often exceed maximum residue limits. Exposure to organophosphorus pesticides through direct contact, ingestion or inhalation can have a deleterious effect on human health ; additional residue in food also contributes to the risk of exposure. The neurological system is particularly susceptible to adverse effects from either direct pesticide exposure or through food. Selected pesticides such as methamidophos, fenamiphos, ethoprophos, phosalone, and acetamiprid are pesticides with possible potential to inhibit acetylcholinesterase (AChE), the essential enzyme for neurotransmission. Disruption of AChE biosynthesis process by certain pesticides has also been evidenced, and was considered as one of the mechanisms for the long-term effects of these compounds. In order to fully understand the action of these pesticides, we investigated their effects and mechanisms on enzymatic activity of both AChE and its analog enzyme butyrylcholinesterase (BChE). The objective of this study was to reveal alterations caused by selected pesticides on the enzymatic activity and expression of AChE in selected cell lines. Moreover, a possible detoxication mechanism was tested, taking into account BChE as endogenous bioscavenger of xenobiotics.

pesticides, acetylcholinesterase, butyrylcholinesterase, inhibition, oximes, bioscavanger

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