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Pregled bibliografske jedinice broj: 1136410

Conformational Transitions of Double-Stranded DNA in Thin Films


Serec, Kristina; Šegedin, Nikola; Krajačić, Maria; Dolanski Babić, Sanja
Conformational Transitions of Double-Stranded DNA in Thin Films // Applied Sciences-Basel, 11 (2021), 5; 2360, 18 doi:10.3390/app11052360 (međunarodna recenzija, članak, znanstveni)


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Naslov
Conformational Transitions of Double-Stranded DNA in Thin Films

Autori
Serec, Kristina ; Šegedin, Nikola ; Krajačić, Maria ; Dolanski Babić, Sanja

Izvornik
Applied Sciences-Basel (2076-3417) 11 (2021), 5; 2360, 18

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA thin films ; FTIR ; hydration ; conformation ; peak fitting ; A-form ; B-form

Sažetak
Conformational transitions of double-stranded DNA in different environments have long been studied as vital parts of both in vitro and in vivo processes. In this study, utilizing Fourier transform infrared spectroscopy (FTIR), we provide detailed analysis of dynamics of A- to B-form transitions in DNA thin films of different hydrated states based on a statistical analysis of a substantial number of spectra and band shape analysis (peak fitting) in both the phosphate (1150–1000 cm􀀀1) and sugar–phosphate (900–750 cm􀀀1) region. Hydration of DNA thin films is systematically controlled by the time spent in the desiccator chamber (from 3 min to 40 min) allowing conformation and hydration signatures, in addition to variations due to ambient conditions, to be resolved in the spectra. Conformation transition from A-form to more ordered B-form is observed if sufficient time in the desiccator chamber is allowed and is confirmed by changes on the bands at 890, 860, 837, and 805 cm􀀀1. Phosphate vibrations at 1230 cm􀀀1 and 1089 cm􀀀1, and backbone vibrations at 1030 cm􀀀1 and 765 cm􀀀1 were found to be sensitive to changes in hydration rather than conformation. Additionally, we found that spectral variations caused by ambient conditions can be significantly reduced without inducing conformational changes, which serves as a good basis for quality assurance.

Izvorni jezik
Hrvatski

Znanstvena područja
Fizika



POVEZANOST RADA


Ustanove:
Medicinski fakultet, Zagreb

Citiraj ovu publikaciju

Serec, Kristina; Šegedin, Nikola; Krajačić, Maria; Dolanski Babić, Sanja
Conformational Transitions of Double-Stranded DNA in Thin Films // Applied Sciences-Basel, 11 (2021), 5; 2360, 18 doi:10.3390/app11052360 (međunarodna recenzija, članak, znanstveni)
Serec, K., Šegedin, N., Krajačić, M. & Dolanski Babić, S. (2021) Conformational Transitions of Double-Stranded DNA in Thin Films. Applied Sciences-Basel, 11 (5), 2360, 18 doi:10.3390/app11052360.
@article{article, year = {2021}, pages = {18}, DOI = {10.3390/app11052360}, chapter = {2360}, keywords = {DNA thin films, FTIR, hydration, conformation, peak fitting, A-form, B-form}, journal = {Applied Sciences-Basel}, doi = {10.3390/app11052360}, volume = {11}, number = {5}, issn = {2076-3417}, title = {Conformational Transitions of Double-Stranded DNA in Thin Films}, keyword = {DNA thin films, FTIR, hydration, conformation, peak fitting, A-form, B-form}, chapternumber = {2360} }
@article{article, year = {2021}, pages = {18}, DOI = {10.3390/app11052360}, chapter = {2360}, keywords = {DNA thin films, FTIR, hydration, conformation, peak fitting, A-form, B-form}, journal = {Applied Sciences-Basel}, doi = {10.3390/app11052360}, volume = {11}, number = {5}, issn = {2076-3417}, title = {Conformational Transitions of Double-Stranded DNA in Thin Films}, keyword = {DNA thin films, FTIR, hydration, conformation, peak fitting, A-form, B-form}, chapternumber = {2360} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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