Degradation Products of Azetidine Core G334089 - Isolation, Structure Elucidation and Pathway (CROSBI ID 296632)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Čikoš, Ana ; Dragojević, Snježana ; Kubiček, Adrijana
engleski
Degradation Products of Azetidine Core G334089 - Isolation, Structure Elucidation and Pathway
An extensive forced degradation study using hydrolytic degradation conditions was performed on G334089, the S-enantiomer of the free fatty acid receptor 2 (FFA2) antagonist GLPG0974, to identify the degradation product structures and discern degradation pathways. Not all degradation products gener- ated ions in the MS spectra, while several others were isomers, so more rigorous degradation conditions were applied to increase the degradant yield. Esterification of the degradants facilitated isolation via preparative HPLC and subsequent NMR and MS characterisation. The determined structures, retention times and fragmentation patterns were used to identify the original degradation products and postu- late a degradation pathway. In addition to the expected amide bond hydrolysis, a second degradation mechanism involving azetidine activation through formation of an azetidinium ion was demonstrated.
degradation products ; azetidine ; GLPG0974 ; FFA2 ; LC-MS ; NMR ; esterification
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Podaci o izdanju
203
2021.
114232
7
objavljeno
0731-7085
1873-264X
10.1016/j.jpba.2021.114232