engleski

GENETIC DIVERSITY AND POPULATION STRUCTURE OF THE NOBLE CRAYFISH IN CROATIA

The noble crayfish Astacus astacus L. is a native European freshwater species whose contemporary distribution and genetics have been shaped by geo-climatic events (i.e. Pleistocene glaciations) and anthropogenic impacts (i.e. translocations, pollution, invasive species). Previous studies revealed that this species harbours highest genetic diversity in the south- eastern Europe, where it survived Pleistocene glaciations [1, 2]. Strong anthropogenic pressure onto its habitats, the presence of non-indigenous invasive crayfish species and climate change are the main reasons for populations decline, therefore the noble crayfish is recognized as threatened and protected by national and international legislation. Maintenance of genetic diversity of a native species in order to protect their adaptive potential is one of the pivotal conservation genetics goals. Guarding in mind declines in population numbers and sizes that cause loss of genetic diversity, we aimed to reveal genetic diversity and structure of the noble crayfish populations in Croatia and identify local genetic hotspots. For that purpose, we used 422 crayfish from 18 populations and 15 recently developed microsatellite loci [3]. Microsatellite analyses revealed moderate level of genetic diversity and differentiation among studied populations reflecting long periods of isolation with restricted gene flow. Moreover, relatively high genetic diversity within populations was recorded. Sampled populations represented two distinct genetic clusters. Results of genetic characterisation will have enabled selection of suitable donor populations for future restocking and/or reintroduction programs. The obtained results help identification of populations and areas with the highest conservation value which should be given the highest priority in protection.

Astacus astacus ; diversity

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