hrvatski

Cytogenetic status of workers exposed to low doses of ionizing radiation with dosimetry and importance of the distribution of DNA damage in different biodosimetry methods in the results estimation

Risk assessment and DNA damage estimation in low doses ionizing radiation (IR) exposure has still many uncertainties, especially among chronically exposed personnel. Different biological assay can be used in bio-dosimetry, but usual strategy is demonstration of only ultimate outcome: damage number/frequency. We demonstrated that besides total number/frequency, damage distribution subcategories can give a better insight in the DNA damage severity as the result of the working life-time exposure. We evaluated DNA damage in 19 occupationally IR low dose exposed medical workers and their controls (19) matched by age, gender and smoking status in fresh whole blood using alkaline comet (TL-tail length, TI-tail intensity), micronucleus cytome (MN, nuclear buds, nucleoplasmic bridges, proliferation index, apoptosis, necrosis), chromosomal aberrations (chromatid/chromosomal breaks, acentric/bicentric frequency) and bleomycin radiomimetic challenging assay (BLM) (breaks frequency, number of metaphases with damage). With 0.58 ± 1.38 mSv (mean ± standard deviation) occupational effective dose, 13.95 ± 8.95 years of exposure, medical workers demonstrated higher DNA damage levels for all assays except BLM. Dose received-DNA damage correlation was seen only in the DNA damage assays sub- categories and not for the total DNA damage number/frequency. These facts suggest that lifetime accumulated DNA damage is not only the result of the IR exposure, and that the truly influence of the dose received during working period of medical workers can be seen by analysing in detail the DNA damage distribution through the categories.

HDZZ ; CRPA ; ionizing radiation ; protection ; micronucleus ; comet assay ; dosimetry ; medical workers

nije evidentirano

engleski

Cytogenetic status of workers exposed to low doses of ionizing radiation with dosimetry and importance of the distribution of DNA damage in different biodosimetry methods in the results estimation

Risk assessment and DNA damage estimation in low doses ionizing radiation (IR) exposure has still many uncertainties, especially among chronically exposed personnel. Different biological assay can be used in bio-dosimetry, but usual strategy is demonstration of only ultimate outcome: damage number/frequency. We demonstrated that besides total number/frequency, damage distribution subcategories can give a better insight in the DNA damage severity as the result of the working life-time exposure. We evaluated DNA damage in 19 occupationally IR low dose exposed medical workers and their controls (19) matched by age, gender and smoking status in fresh whole blood using alkaline comet (TL-tail length, TI-tail intensity), micronucleus cytome (MN, nuclear buds, nucleoplasmic bridges, proliferation index, apoptosis, necrosis), chromosomal aberrations (chromatid/chromosomal breaks, acentric/bicentric frequency) and bleomycin radiomimetic challenging assay (BLM) (breaks frequency, number of metaphases with damage). With 0.58 ± 1.38 mSv (mean ± standard deviation) occupational effective dose, 13.95 ± 8.95 years of exposure, medical workers demonstrated higher DNA damage levels for all assays except BLM. Dose received-DNA damage correlation was seen only in the DNA damage assays sub- categories and not for the total DNA damage number/frequency. These facts suggest that lifetime accumulated DNA damage is not only the result of the IR exposure, and that the truly influence of the dose received during working period of medical workers can be seen by analysing in detail the DNA damage distribution through the categories.

HDZZ ; CRPA ; ionizing radiation ; protection ; micronucleus ; comet assay ; dosimetry ; medical workers

nije evidentirano