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Pregled bibliografske jedinice broj: 1131733

Who's in, who's out? Re-evaluation of lipid raft residents


Mlinac Jerković, Kristina; Ilić, Katarina; Zjalić, Milorad; Mandić, Dario; Debeljak, Željko; Balog, Marta; Damjanović, Vladimir; Maček Hrvat, Nikolina; Habek, Nikola; Kalanj Bognar, Svjetlana et al.
Who's in, who's out? Re-evaluation of lipid raft residents // Journal of neurochemistry, 158 (2021), 3; 657-672 doi:10.1111/jnc.15446 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1131733 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Who's in, who's out? Re-evaluation of lipid raft residents

Autori
Mlinac Jerković, Kristina ; Ilić, Katarina ; Zjalić, Milorad ; Mandić, Dario ; Debeljak, Željko ; Balog, Marta ; Damjanović, Vladimir ; Maček Hrvat, Nikolina ; Habek, Nikola ; Kalanj Bognar, Svjetlana ; Schnaar, Ronald L. ; Heffer, Marija

Izvornik
Journal of neurochemistry (0022-3042) 158 (2021), 3; 657-672

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
detergent-resistant membranes ; glycosphingolipids ; glutamate receptor subunit 2 ; amyloid precursor protein ; neuroplastin

Sažetak
Lipid rafts, membrane microdomains enriched with (glyco)sphingolipids, cholesterol, and select proteins, act as cellular signalosomes. Various methods have been used to separate lipid rafts from bulk (non-raft) membranes, but most often non-ionic detergent Triton X-100 has been used in their isolation. However, Triton X-100 is a reported disruptor of lipid rafts. Histological evidence confirmed raft disruption by Triton X-100 but remarkably revealed raft stability to treatment with a related polyethylene oxide detergent, Brij O20. We report isolation of detergent-resistant membranes from mouse brain using Brij O20 and its use to determine the distribution of major mammalian brain gangliosides, GM1, GD1a, GD1b and GT1b. A different distribution of gangliosides – classically used as a raft marker - was discovered using Brij O20 versus Triton X-100. Immunohistochemistry and imaging mass spectrometry confirm the results. Use of Brij O20 results in a distinctive membrane distribution of gangliosides that is not all lipid raft associated but depends on the ganglioside structure. This is the first report of a significant proportion of gangliosides outside raft domains. We also determined the distribution of proteins functionally related to neuroplasticity and known to be affected by ganglioside environment, glutamate receptor subunit 2, amyloid precursor protein and neuroplastin and report the lipid raft populations of these proteins in mouse brain tissue. This work will enable more accurate lipid raft analysis with respect to glycosphingolipid and membrane protein composition and lead to improved resolution of lipid-protein interactions within biological membranes.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
IP-2016-06-8636 - Molekularni biljezi vulnerabilnosti, adaptacije i plastičnosti neurona u akutnoj i kroničnoj ozljedi mozga (NeuroReact) (Kalanj Bognar, Svjetlana, HRZZ - 2016-06) ( POIROT)
HRZZ-IP-2014-09-2324 - Patofiziološke posljedice promjena sastava lipidnih splavi (RafTuning) (Heffer, Marija, HRZZ - 2014-09) ( POIROT)
EK-EFRR-KK.01.1.1.01.0007 - Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi (Kostović, Ivica, EK - KK.01.1.1.01) ( POIROT)
EK-EFRR-KK.01.1.1.02.0015 - Istraživanje i dijagnostika malignih, infektivnih i rijetkih metaboličkih bolesti temeljena na MALDI TOF tehnologiji (Debeljak, Željko, EK - KK.01.1.1.02) ( POIROT)

Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Medicinski fakultet, Zagreb,
Medicinski fakultet, Osijek

Poveznice na cjeloviti tekst rada:

doi onlinelibrary.wiley.com onlinelibrary.wiley.com

Citiraj ovu publikaciju:

Mlinac Jerković, Kristina; Ilić, Katarina; Zjalić, Milorad; Mandić, Dario; Debeljak, Željko; Balog, Marta; Damjanović, Vladimir; Maček Hrvat, Nikolina; Habek, Nikola; Kalanj Bognar, Svjetlana et al.
Who's in, who's out? Re-evaluation of lipid raft residents // Journal of neurochemistry, 158 (2021), 3; 657-672 doi:10.1111/jnc.15446 (međunarodna recenzija, članak, znanstveni)
Mlinac Jerković, K., Ilić, K., Zjalić, M., Mandić, D., Debeljak, Ž., Balog, M., Damjanović, V., Maček Hrvat, N., Habek, N. & Kalanj Bognar, S. (2021) Who's in, who's out? Re-evaluation of lipid raft residents. Journal of neurochemistry, 158 (3), 657-672 doi:10.1111/jnc.15446.
@article{article, author = {Mlinac Jerkovi\'{c}, Kristina and Ili\'{c}, Katarina and Zjali\'{c}, Milorad and Mandi\'{c}, Dario and Debeljak, \v{Z}eljko and Balog, Marta and Damjanovi\'{c}, Vladimir and Ma\v{c}ek Hrvat, Nikolina and Habek, Nikola and Kalanj Bognar, Svjetlana and Schnaar, Ronald L. and Heffer, Marija}, year = {2021}, pages = {657-672}, DOI = {10.1111/jnc.15446}, keywords = {detergent-resistant membranes, glycosphingolipids, glutamate receptor subunit 2, amyloid precursor protein, neuroplastin}, journal = {Journal of neurochemistry}, doi = {10.1111/jnc.15446}, volume = {158}, number = {3}, issn = {0022-3042}, title = {Who's in, who's out? Re-evaluation of lipid raft residents}, keyword = {detergent-resistant membranes, glycosphingolipids, glutamate receptor subunit 2, amyloid precursor protein, neuroplastin} }
@article{article, author = {Mlinac Jerkovi\'{c}, Kristina and Ili\'{c}, Katarina and Zjali\'{c}, Milorad and Mandi\'{c}, Dario and Debeljak, \v{Z}eljko and Balog, Marta and Damjanovi\'{c}, Vladimir and Ma\v{c}ek Hrvat, Nikolina and Habek, Nikola and Kalanj Bognar, Svjetlana and Schnaar, Ronald L. and Heffer, Marija}, year = {2021}, pages = {657-672}, DOI = {10.1111/jnc.15446}, keywords = {detergent-resistant membranes, glycosphingolipids, glutamate receptor subunit 2, amyloid precursor protein, neuroplastin}, journal = {Journal of neurochemistry}, doi = {10.1111/jnc.15446}, volume = {158}, number = {3}, issn = {0022-3042}, title = {Who's in, who's out? Re-evaluation of lipid raft residents}, keyword = {detergent-resistant membranes, glycosphingolipids, glutamate receptor subunit 2, amyloid precursor protein, neuroplastin} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





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