engleski

Kinetic model of halohydrin dehalogenase-catalysed synthesis of (R)-ECH

Halohydrin dehalogenases (HHDHs) are valuable and versatile biocatalysts. They can easily be obtained in large quantities by expression in recombinant E. coli cells1. HHDH enzymes are divided into subtypes A - G and show promising properties in stereoselective biocatalysis but are still insufficiently investigated1. HHDHs are industrially applied for the synthesis of chiral epichlorohydrin (ECH). ECH is widely used as a starting material in the production of insecticides, surfactants, adhesives, pharmaceuticals, etc1. Main disadvantages of the traditional synthesis of chiral ECH is use of environmentally unfriendly, metal catalyst (cobalt-salen complexes) and relatively low reaction yield as it is limited at 50 %. Biocatalytic methods for chiral ECH preparation are getting much attention mostly because of environmentally friendly conditions, extensive enzyme sources, high enantioselectivity and low production cost2. Several studies involving the synthesis of chiral ECH by HHDH mutants have been published3-5. We have developed kinetic model of HheB2-T120A-catalysed synthesis of (R)-ECH from 1, 3-dichloro-2-propanol. Kinetic characterisation offers better understanding of reaction and is an essential step in the development of mathematical model which ultimately helps with the optimization of the reaction conditions.

Halohydrin dehalogenase ; (R)-epichlorohydrin

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