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Pregled bibliografske jedinice broj: 1131480

Characterization of gross genome rearrangements in Deinococcus radiodurans recA mutants


Repar, Jelena; Zahradka, Davor; Sović, Ivan; Zahradka, Ksenija
Characterization of gross genome rearrangements in Deinococcus radiodurans recA mutants // Scientific reports, 11 (2021), 1; 10939, 11 doi:10.1038/s41598-021-89173-9 (međunarodna recenzija, članak, znanstveni)


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Naslov
Characterization of gross genome rearrangements in Deinococcus radiodurans recA mutants

Autori
Repar, Jelena ; Zahradka, Davor ; Sović, Ivan ; Zahradka, Ksenija

Izvornik
Scientific reports (2045-2322) 11 (2021), 1; 10939, 11

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
genome stability ; gamma-radiation ; DNA double-strand break repair ; alternative end-joining

Sažetak
Genome stability in radioresistant bacterium Deinococcus radiodurans depends on RecA, the main bacterial recombinase. Without RecA, gross genome rearrangements occur during repair of DNA double- strand breaks. Long repeated (insertion) sequences have been identified as hot spots for ectopic recombination leading to genome rearrangements, and single-strand annealing (SSA) postulated to be the most likely mechanism involved in this process. Here, we have sequenced five isolates of D. radiodurans recA mutant carrying gross genome rearrangements to precisely characterize the rearrangements and to elucidate the underlying repair mechanism. The detected rearrangements consisted of large deletions in chromosome II in all the sequenced recA isolates. The mechanism behind these deletions clearly differs from the classical SSA ; it utilized short (4–11 bp) repeats as opposed to insertion sequences or other long repeats. Moreover, it worked over larger linear DNA distances from those previously tested. Our data are most compatible with alternative end- joining, a recombination mechanism that operates in eukaryotes, but is also found in Escherichia coli. Additionally, despite the recA isolates being preselected for different rearrangement patterns, all identified deletions were found to overlap in a 35 kb genomic region. We weigh the evidence for mechanistic vs. adaptive reasons for this phenomenon.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
IP-2013-11-2978 - Rekombinacija, popravak DNA i očuvanje integriteta genoma: novi putevi (RECNEWPATH) (Zahradka, Davor, HRZZ - 2013-11) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Davor Zahradka (autor)

Avatar Url Ksenija Zahradka (autor)

Avatar Url Ivan Sović (autor)

Avatar Url Jelena Repar (autor)

Citiraj ovu publikaciju

Repar, Jelena; Zahradka, Davor; Sović, Ivan; Zahradka, Ksenija
Characterization of gross genome rearrangements in Deinococcus radiodurans recA mutants // Scientific reports, 11 (2021), 1; 10939, 11 doi:10.1038/s41598-021-89173-9 (međunarodna recenzija, članak, znanstveni)
Repar, J., Zahradka, D., Sović, I. & Zahradka, K. (2021) Characterization of gross genome rearrangements in Deinococcus radiodurans recA mutants. Scientific reports, 11 (1), 10939, 11 doi:10.1038/s41598-021-89173-9.
@article{article, year = {2021}, pages = {11}, DOI = {10.1038/s41598-021-89173-9}, chapter = {10939}, keywords = {genome stability, gamma-radiation, DNA double-strand break repair, alternative end-joining}, journal = {Scientific reports}, doi = {10.1038/s41598-021-89173-9}, volume = {11}, number = {1}, issn = {2045-2322}, title = {Characterization of gross genome rearrangements in Deinococcus radiodurans recA mutants}, keyword = {genome stability, gamma-radiation, DNA double-strand break repair, alternative end-joining}, chapternumber = {10939} }
@article{article, year = {2021}, pages = {11}, DOI = {10.1038/s41598-021-89173-9}, chapter = {10939}, keywords = {genome stability, gamma-radiation, DNA double-strand break repair, alternative end-joining}, journal = {Scientific reports}, doi = {10.1038/s41598-021-89173-9}, volume = {11}, number = {1}, issn = {2045-2322}, title = {Characterization of gross genome rearrangements in Deinococcus radiodurans recA mutants}, keyword = {genome stability, gamma-radiation, DNA double-strand break repair, alternative end-joining}, chapternumber = {10939} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • Social Science Citation Index (SSCI)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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