Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 1130682

Interaction of Differently Sized, Shaped, and Functionalized Silver and Gold Nanoparticles with Glycosylated versus Nonglycosylated Transferrin


Barbir, Rinea; Ramírez Jiménez, Rafael; Martín-Rapún, Rafael; Strasser, Vida; Domazet Jurašin, Darija; Dabelić, Sanja; de la Fuente, Jesus M.; Vinković Vrček, Ivana
Interaction of Differently Sized, Shaped, and Functionalized Silver and Gold Nanoparticles with Glycosylated versus Nonglycosylated Transferrin // ACS applied materials & interfaces (2021) doi:10.1021/acsami.1c04063 (znanstveni, online first)


CROSBI ID: 1130682 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Interaction of Differently Sized, Shaped, and Functionalized Silver and Gold Nanoparticles with Glycosylated versus Nonglycosylated Transferrin

Autori
Barbir, Rinea ; Ramírez Jiménez, Rafael ; Martín-Rapún, Rafael ; Strasser, Vida ; Domazet Jurašin, Darija ; Dabelić, Sanja ; de la Fuente, Jesus M. ; Vinković Vrček, Ivana

Vrsta, podvrsta
Radovi u časopisima, znanstveni

Izvornik
ACS applied materials & interfaces (2021)

Status rada
Online first

Ključne riječi
silver gold transferrin albumin protein binding conformational changes

Sažetak
Exposure of nanomaterials (NMs) to biological medium results in their direct interaction with biomolecules and the formation of a dynamic biomolecular layer known as the biomolecular corona. Despite numerous published data on nano-biointeractions, the role of protein glycosylation in the formation, characteristics, and fate of such nano-biocomplexes has been almost completely neglected, although most serum proteins are glycosylated. This study aimed to systematically investigate the differences in interaction of metallic NPs with glycosylated vs nonglycosylated transferrin. To reach this aim, we compared interaction mechanisms between differently sized, shaped, and surface-functionalized silver NMs and gold NMs to commercially available human transferrin (TRF), a glycosylated protein, and to its nonglycosylated recombinant form (ngTRF). Bovine serum albumin (BSA) was also included in the study for comparative purposes. Characterization of NMs was performed using transmission electron microscopy and dynamic and electrophoretic light scattering techniques. Fluorescence quenching and circular dichroism methods were used to evaluate protein binding constants on the nanosurface and conformational changes after the protein–NM interactions, respectively. Competitive binding of TRF, ngTRF, and BSA to the surface of different NMs was evaluated by separating them after extraction from protein corona by gel electrophoresis following quantification with a commercial protein assay. The results showed that the binding strength between NMs and transferrin and the changes in the secondary protein structure largely depend not only on NM physicochemical properties but also on the protein glycosylation status. Data gained by this study highlight the relevance of protein glycosylation for all future design, development, and efficacy and safety assessment of NMs.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Interdisciplinarne prirodne znanosti, Farmacija



POVEZANOST RADA


Projekti:
HRZZ-PZS-2019-02-4323 - Siguran pristup za razvoj nano-sustava za ciljanu isporuku lijekova u mozak (SENDER) (Vinković Vrček, Ivana, HRZZ - 2019-02) ( POIROT)
IP-2016-06-2436 - Značaj interakcija metalnih nanočestica sa sumpornim biomolekulama za nano-bio sučelje (NanoFaceS) (Vinković Vrček, Ivana, HRZZ - 2016-06) ( POIROT)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Institut "Ruđer Bošković", Zagreb

Citiraj ovu publikaciju

Barbir, Rinea; Ramírez Jiménez, Rafael; Martín-Rapún, Rafael; Strasser, Vida; Domazet Jurašin, Darija; Dabelić, Sanja; de la Fuente, Jesus M.; Vinković Vrček, Ivana
Interaction of Differently Sized, Shaped, and Functionalized Silver and Gold Nanoparticles with Glycosylated versus Nonglycosylated Transferrin // ACS applied materials & interfaces (2021) doi:10.1021/acsami.1c04063 (znanstveni, online first)
Barbir, R., Ramírez Jiménez, R., Martín-Rapún, R., Strasser, V., Domazet Jurašin, D., Dabelić, S., de la Fuente, J. & Vinković Vrček, I. (2021) Interaction of Differently Sized, Shaped, and Functionalized Silver and Gold Nanoparticles with Glycosylated versus Nonglycosylated Transferrin. Prihvaćen za objavljivanje u ACS applied materials & interfaces. [Preprint] doi:10.1021/acsami.1c04063.
@unknown{unknown, year = {2021}, DOI = {10.1021/acsami.1c04063}, keywords = {silver gold transferrin albumin protein binding conformational changes}, journal = {ACS applied materials and interfaces}, doi = {10.1021/acsami.1c04063}, title = {Interaction of Differently Sized, Shaped, and Functionalized Silver and Gold Nanoparticles with Glycosylated versus Nonglycosylated Transferrin}, keyword = {silver gold transferrin albumin protein binding conformational changes} }
@unknown{unknown, year = {2021}, DOI = {10.1021/acsami.1c04063}, keywords = {silver gold transferrin albumin protein binding conformational changes}, journal = {ACS applied materials and interfaces}, doi = {10.1021/acsami.1c04063}, title = {Interaction of Differently Sized, Shaped, and Functionalized Silver and Gold Nanoparticles with Glycosylated versus Nonglycosylated Transferrin}, keyword = {silver gold transferrin albumin protein binding conformational changes} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font