engleski

Determination of Clenbuterol in Pig Liver Following Prolonged Administration of a Growth-Promoting Dose

Liver concentration of clenbuterol, a beta-adrenergic agonist, were measured by enzyme-linked immunosorbent assay (ELISA) in 12 female pigs on days 0, 7, 14 and 21 after prolonged administration of growth-promoting dose of clenbuterol. The analytical procedure showed good recovery, while intra-assay results showed acceptable variation in individual measuraments for all samples to which 0.5, 2 or 5 ng/g clenbuterol were added. The assay detection limit was 0.13 ng/g and quantification limit 0.16 ng/g.The results indicated that 80% clenbuterol depletion in pig liver occurred during the first 7 days after cessation of administration, however, more than 14 days were required for the clenbuterol concentration to fall below the maximal residual level of 0.5 ng/g. On day 21 of clenbuterol discontinuation, residues of the drug were detectable in pig liver, suggesting that clenbuterol residues persist in liver as an edible tissue and may induce a risk for consumer health.

beta-adrenergic agonist; clenbuterol; anabolic agent; enzyme immunoassay; pig liver

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