engleski

Changes in serum proteome in canine pyometra

Introduction: Pyometra is the most common disease of the uterus in intact adult bitches after non- pregnant oestrous cycles. This disease can develop a wide range of clinical signs from subclinical to a fatal illness, which could make the early diagnostic of pyometra challenging, especially when there is no vaginal discharge. Objectives: To compare the serum proteome in healthy bitches and bitches with pyometra through a highthroughput quantitative proteomic analysis. The differentially expressed proteins could reflect the physiopathological changes occurring in canine pyometra and may be considered as potential biomarkers of the disease. Methods: Serum sample surplus left after routine serum analysis was used. In any case, additional sample was extracted from dogs for this study. Serum samples from six healthy (H) and six client-owned bitches with pyometra (P) were analysed by Tandem Mass Tags (TMT) analysis. Results: Proteomic analysis quantified 389 proteins in serum. When the two groups were compared, three unique proteins were modulated: keratin type I were down-regulated ; while serotransferrin and immunoglobin A were up- regulated in canine pyometra. Conclusions: There are changes in serum proteome in bitches with pyometra in comparison to healthy ones. Keratin type I, serotransferrin, and immunoglobulin A have potential as biomarkers of diagnosis in this disease.

tandem mass tag ; proteomics ; serum ; canine pyometra

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