engleski

The salivary proteome in canine pyometra

Introduction: Pyometra is the most common disease of the uterus in intact adult bitches. It consists of a suppurative bacterial infection of the uterus which may cause accumulation of inflammatory exudate and produces a variety of clinical manifestations. Objectives: To evaluate the changes in the salivary proteome in bitches with pyometra through a highthroughput quantitative proteomic analysis with the aims to explore whether saliva composition could reflect the physiopathological changes occurring in canine pyometra. Methods: Saliva samples from client-owned dogs were collected for diagnostic purposes, divided into two groups: healthy (H, n=6) and bitches with pyometra (P, n=6), and analysed using Tandem Mass Tags (TMT) - based approach. Results: Proteomic analysis quantified 707 proteins in saliva. Comparison of two groups revealed 16 unique proteins significantly modulated in saliva. According to Protein Analysis Through Evolutionary Relationships (PANTHER) classification tool, these proteins belong to two molecular functions: catalytic activity (80%) and binding (20%). Six different biological processes were involved, being metabolic and cellular process the majoritarian with 39.9 and 33.3% of proteins, respectively. Six biological pathways were involved, being blood coagulation, integrin signalling pathway, and plasminogen activating cascade the most represented with 22.2% of proteins each. Finally, in relation with protein class, 41% were hydrolases, followed by oxidoreductases (25%). Conclusions: There are changes in various proteins in saliva in canine pyometra reflecting different physiopathological changes occurring in this disease. These proteins could be a source of potential non-invasive biomarkers for this disease that should be confirmed in future studies.

Canine pyometra ; TMT-based proteomics ; Saliva

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