hrvatski

Ljudski papiloma virus kod karcinoma pluća nemalih stanica s mutacijama gena EGFR

Activating mutations/deletions in the epidermal growth factor receptor (EGFR) gene are present in 15% of patients diagnosed with non-small cell lung cancer (NSCLC). Exon 19 deletions and codon 858 point mutations of exon 21 are the most common mutations in NSCLC. The presence of point mutations, deletions and insertions within exons 18 - 25 is responsible for a poor response to tyrosine kinase inhibitors. Types 16 and 18 of human papillomavirus (HPV) are considered to be of high risk for cervical cancer development and progression. The aim of this study was to determine the prevalence of HPV infection in NSCLC cells in relation to the presence and frequency of EGFR gene mutations. In this study, 34 NSCLC samples with EGFR gene mutations and 33 NSCLC samples without EGFR gene mutations were analyzed. Furthermore, EGFR gene mutations were divided into classical (ex19del ; L858R) and rare (G719X ; ex20ins ; S768I ; T790M and ex21). Fragments of the E7 gene were amplified by PCR for HPV16 and HPV18 DNA detection. The possible higher prevalence of HPV infection in NSCLC patients with EGFR mutations, compared to NSCLC patients without EGFR mutations, was examined by meta-analysis using data from this and six other studies. More frequent HPV infection was observed in the NSCLC with EGFR gene mutations (HPV16, n=11, p<0.05 ; HPV18, n=24, p<0.001) compared to the NSCLC without EGFR gene mutations. Significant association between smoking and HPV16 infection was observed (p<0.028) in the NSCLC with EGFR gene mutations. Among 34 NSCLC samples, 46 mutations in the EGFR gene were detected. Classical EGFR gene mutations were detected in 20 (58.8%) NSCLC samples and rare mutations in five (14.7%) samples. Nine (26.5%) NSCLC samples had a combination of classical and rare EGFR gene mutations. The significant association between the presence of rare EGFR gene mutations and HPV16 and/or HPV18 infection (p<0.05) was observed. A meta-analysis confirmed more frequent HPV infection in the NSCLC with EGFR gene mutations. The obtained results indicate the need for further research into the role of HPV in the pathogenesis of NSCLC with EGFR gene mutations.

ljudski papiloma virus ; karcinom pluća nemalih stanica ; mutacija gena EGFR

nije evidentirano

engleski

Human papillomavirus in non-small cell lung cancer with EGFR gene mutations

Activating mutations/deletions in the epidermal growth factor receptor (EGFR) gene are present in 15% of patients diagnosed with non-small cell lung cancer (NSCLC). Exon 19 deletions and codon 858 point mutations of exon 21 are the most common mutations in NSCLC. The presence of point mutations, deletions and insertions within exons 18 - 25 is responsible for a poor response to tyrosine kinase inhibitors. Types 16 and 18 of human papillomavirus (HPV) are considered to be of high risk for cervical cancer development and progression. The aim of this study was to determine the prevalence of HPV infection in NSCLC cells in relation to the presence and frequency of EGFR gene mutations. In this study, 34 NSCLC samples with EGFR gene mutations and 33 NSCLC samples without EGFR gene mutations were analyzed. Furthermore, EGFR gene mutations were divided into classical (ex19del ; L858R) and rare (G719X ; ex20ins ; S768I ; T790M and ex21). Fragments of the E7 gene were amplified by PCR for HPV16 and HPV18 DNA detection. The possible higher prevalence of HPV infection in NSCLC patients with EGFR mutations, compared to NSCLC patients without EGFR mutations, was examined by meta-analysis using data from this and six other studies. More frequent HPV infection was observed in the NSCLC with EGFR gene mutations (HPV16, n=11, p<0.05 ; HPV18, n=24, p<0.001) compared to the NSCLC without EGFR gene mutations. Significant association between smoking and HPV16 infection was observed (p<0.028) in the NSCLC with EGFR gene mutations. Among 34 NSCLC samples, 46 mutations in the EGFR gene were detected. Classical EGFR gene mutations were detected in 20 (58.8%) NSCLC samples and rare mutations in five (14.7%) samples. Nine (26.5%) NSCLC samples had a combination of classical and rare EGFR gene mutations. The significant association between the presence of rare EGFR gene mutations and HPV16 and/or HPV18 infection (p<0.05) was observed. A meta-analysis confirmed more frequent HPV infection in the NSCLC with EGFR gene mutations. The obtained results indicate the need for further research into the role of HPV in the pathogenesis of NSCLC with EGFR gene mutations.

human papillomavirus ; non-small cell lung cancer ; EGFR gene mutations

nije evidentirano