engleski

HLA TYPING STRATEGY IN THE CROATIAN BONE MARROW DONOR REQISTRY: GENDER AND AGE ASPECT

Hematopoietic stem cell donor registries implement different strategies for improvement of their work, mainly focusing on increasing resolution of HLA typing and number of tested HLA loci on one hand, and young male donor recruitment on the other. The aim of this analysis was to assess the Croatian Bone Marrow Donor Registry (CBMDR) strategy, from the HLA diversity point of view, of age and gender based donor inclusion in the set appropriated to HLA typing by a nextgeneration sequencing method. The CBMDR currently lists >56, 000 donors, with prevalence of female (F) donors both in the total number of CBMDR donors (N(F)=33, 963 ; 60.3%) as well as in different age groups: 18-30yrs (F-62.2%) ; 31-50 yrs (F-60.1%) ; >50yrs (F- 57.7%). HLA phenotypes at HLA-A, -B, -DRB1 loci split level (excluding HLA-B*14, -B*15 and -B*40) were analyzed. The number of different HLA phenotypes discovered in the total number of CBMDR donors is 36, 270 ; which represents 0.27% of all possible combinations. Two HLA phenotypes were seen in >100 donors, 235 HLA phenotypes were observed frequently (11-100x) and 8, 237 HLA phenotypes were detected more sporadically (2-10x). The remaining 27, 796 different HLA phenotypes appeared only once, representing a group of unique HLA phenotypes. The distribution of unique HLA phenotypes in different age groups did not provide evidence of one group being more heterogeneous, since approx. 50% of donors in each group carried a unique HLA phenotype. Similar results were obtained when analysis was extended on female and male donors within the same age group. Finally, analysis of the number of donors carrying unique HLA phenotypes originating from different regions of Croatia also revealed no significant differences. To conclude, based solely on HLA phenotype diversity perspective, each donor group, regardless of the age, gender and origin, contributes equally to HLA phenotype variety of CBMDR, and should be included in future highresolution HLA typing.

HLA typing, CBMDR

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