engleski

In a rat model of ageing, long term treatment with wine antioxidant, resveratrol, upregulates plasma levels of testosterone in males and downregulates plasma levels of progesterone in females

Introduction: Aging-related impaired body structure and functions are assumed to be, at least partially, caused by elevated oxidative stress (OS). A red wine polyphenol resveratrol may act as an antioxidant and anti-aging medium, but its actions in vivo are controversial (1). Here we report on sex- related effects of resveratrol treatment (RT) on blood/plasma parameters of DNA damage, oxidative status, and concentrations of sex hormones (SH) in a rat model of aging (2). Material & Methods: Starting from their age of 3-mo, for the next 9-mo or 21-mo male and female Wistar rats were drinking resveratrol in water (10 mg/L ; predicted intake: ~1 mg/kg b.m./day) or only water (controls). In their blood/plasma we performed the alkaline comet assay (3) and determined glutathione (4) and malondialdehyde (5) by the established methods. SH were determined by commercial kits. Results: Compared with controls, the 9-mo RT did not change any parameter in both sexes. After the 21-mo RT, the OS-related parameters remained unchanged, but testosterone levels in males were ⁓45% higher (P<0.001), and progesterone levels in females were ⁓40% (P<0.01) lower. Conclusion: In old rats, a long-term RT does not change the OS-related parameters in blood/plasma, but it affects the levels of SH. References 1. J. Gambini et al. Properties of resveratrol: in vitro and in vivo studies about metabolism, bioavailability, and biological effects in animal models and humans. Oxid. Med. Cell. Longev., Volume 2015, Article ID 837042, 13 pages, http://dx.doi.org/10.1155/2015/837042 2. Croatian Science Foundation project IP- 2013-11-1481 3. N.P. Singh et al. A simple technique for quantitation of low levels of DNA damage in individual cells, Exp. Cell Res. 175 (1988) 184–191. 4. G.L. Ellman. A colorimetric method for determining low concentrations of mercaptans, Arch. Biochem. Biophys. 74 (1958) 443-450. 5. J.A. Drury et al. Comparison of urinary and plasma malondialdehyde in preterm infants, Clin. Chim. Acta 263 (1997) 177-185.

Rat ; Resveratrol ; Testosterone ; Plasma

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