engleski

Screening of Croatian Native Grapevine Varieties for Susceptibility to Plasmopara viticola Using Leaf Disc Bioassay, Chlorophyll Fluorescence, and Multispectral Imaging

In the era of sustainable grapevine production, there is a growing demand to define differences between Vitis vinifera varieties in susceptibility to downy mildew. Croatia, as a country with a long tradition of grapevine cultivation, preserves a large number of native grapevine varieties. A leaf disc bioassay has been conducted on 25 of them to define their response to downy mildew, according to the OIV descriptor 452-1 together with the stress response of the leaf discs using chlorophyll fluorescence and multispectral imaging with eleven parameters included. Time points of measurement were as follows: before treatment (T0), one day post-inoculation (dpi) (T1), two dpi (T2), three dpi (T3), four dpi (T4), six dpi (T5) and eight dpi (T6). Visible changes in form of developed P. viticola sporulation were evaluated on the 7th day upon inoculation. Results show that methods applied here distinguish varieties of different response to downy mildew. Based on the results obtained, a phenotyping model in the absence of the pathogen is proposed, which is required to confirm by conducting more extensive research.

Vitis vinifera L. ; downy mildew ; biotic stress ; chlorophyll fluorescence ; spectral indices ; imaging methodology ; phenotyping model

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