engleski

Screening for grapevine viruses and GLRaV-3 genetic diversity in the most appreciated Croatian red indigenous cultivar Plavac Mali

Plavac Mali is the most important Croatian grapevine cultivar used for red wine production. A direct descendant of Tribidrag also known as Zinfandel and Primitivo, this cultivar is highly appreciated for viticultural practice due to particular adaptation to drought and resistance to various pathogens. Grapevine itself can be affected by more than 70 viruses, some of which are considered to be particularly dangerous to the plant and therefore included in the certification programs of many countries. In this study 35 vines of cv.Plavac Mali from collection vineyard of Institute for Adriatic Crops originating from 13 different locations, were tested for the presence of 10 economically important viruses. Detection for Grapevine leafroll associated virus -1, -2, -3, (GLRaV-1, -2, -3), Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine rupestris stem-pitting associated virus (GRSPaV), Grapevine fanleaf virus (GFLV), Arabis mosaic virus (ArMV) and Grapevine fleck virus (GFkV) was performed using a RT-PCR multiplex reaction (Gambino and Gribaudo 2006). Grapevine pinot gris virus (GPGV) was tested in individual PCR reaction as described by Saldarelli et al. 2015. The highest occurrence of individual virus was the one of GLRaV-3, detected in 85.71% of samples. The following were GVA with 77.14% and GRSPaV with 40 % and the lowest occurrence was the one of GVB (5.71 %). As for coinfections with multiple viruses plants infected with four and five viruses represent the majority of plant population (more than 50% of population tested). Further investigation was performed on GLRaV-3s HSP70h sequence variants (Turturo et al., 2005). Nine vines were chosen for SSCP analysis and phylogenetic groups I and II were detected showing rather low GLRaV-3 genetic diversity. Monophyletic infections (77.78%) prevailed over polyphyletic (22.22%) and in monophyletic infections Group II was dominant over Group I. This study confirms deteriorating sanitary status of Croatian most valuable red indigenous variety and low genetic diversity of GLRaV-3 isolates.

grapevine viruses, indigenous cultivar, leafroll, screening, molecular characterization

nije evidentirano