engleski

Antimicrobial effect of cold atmospheric plasma produced by dielectric barrier discharge against Enterococcus faecalis

Aim To evaluate the antibacterial effect of the atmospheric-pressure dielectric barrier discharge cold atmospheric plasma on suspension of Enterococcus faecalis in planktonic form inoculated in prepared canals of extracted human teeth. Methodology Cold atmospheric plasma field was produced by CR glass probe of high frequency ozone generator Ozonytron X (Biozonix GmbH, Munich, Germany) by dielectric barrier discharge through the ambient air at atmospheric pressure. Thirty extracted human single rooted teeth stored in sterile isotonic saline solution were selected according to size and shape similarities. All teeth were then resected 13 mm from the apex using a diamond bur TR-11 ISO199/016 (Mani, Utsunomiya, Tochigi, Japan) and embedded in acrylic Palavit G resin (Heraeus Kulzer GmbH, Wehrheim, Germany). Their canals were instrumented using the ProTaper rotary instrumentation technique using conventional sequence (S1, SX, S1, S2, F1, F2, F3) according to the manufacturer’s instruction (Maillefer, Ballaigues, Switzerland). Teeth were divided in one experimental group (20 teeth) and two control groups (positive and negative, five teeth each). Before starting each of the experiment cycles, the sterility of the samples was determined. The microorganism used was Enterococcus faecalis ATCC 29212 (LGC-ATCC, Wesel, Germany). Suspension was prepared in BrainHeart (BH) infusion broth with concentration of 109 CFU (Colony Forming Units) and 0.02 ml of mixture of bacterial suspension was inserted into root canals by syringe. The active part of floating CR glass probe was placed at a distance of 1mm from orifice to the prepared root canal and activated at the highest level (5) for 20 and 60 seconds distance. After treatment, root canals have been refilled with 0.02 ml of sterile BH. Results Significant reduction in the range of 108 CFU of Enterococcus faecalis after 20/60 seconds was estimated. Data were analyzed using Wilcoxon paired samples test, a non- parametric test for paired data. Although it has been determined, the difference antimicrobial effect after 20 and 60 seconds was not statistically significant. Conclusions The use of the atmospheric-pressure dielectric barrier discharge cold atmospheric plasma showed pronounced antimicrobial effect against Enterococcus faecalis in planktonic form.

antibacterial effect ; cold atmospheric plasma

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