engleski

Fluorescence spectroscopic study of the interaction of differently coated gold nanoparticles with dopamine and l-dopa

Efficient and safe treatment of patients suffering from Parkinson’s disease possess many challenges. Common therapy that includes external administration of L-dopa to Parkinson’s disease patients is limited due to poor blood-brain barrier (BBB) penetration and lack of targeting ability [1-2]. To overcome these issues, gold nanoparticles (AuNPs) represent an attractive platform for targeted drug delivery into the brain, as they enter the central nervous system (CNS) by penetration through the BBB [3-4]. For this purpose, AuNPs functionalized with polyethylene glycol (PEG), adamantane derivatives, and peptidoglycan monomer (PGM) were prepared[5].In this study, the ability of novel AuNPs to be used as delivery systems for dopamine and L-dopa was evaluated considering their binding capabilities. Steady-state fluorescence spectroscopy was employed to investigate the binding interaction of dopamine and L-dopa on differently coated AuNPs. The strongest interaction was observed between L-dopa/dopamine and AuNPs functionalized with PGM. The intermolecular forces between L-dopa or dopamine and differently coated AuNPs were estimated by thermodynamic analysis. The binding predominantly occurred through Van der Waals interactions or hydrogen bonds, while positive entropy change that is indicative for electrostatic interactions was seen only in a few instances. In all cases, interactions were exothermic and spontaneous since enthalpy change and Gibbs free energy were negative, respectively.

nanoparticles ; gold ; dopa ; dopamine

nije evidentirano