Impact of serum albumin on binding ability of dopamine and L-dopa on differently coated gold nanoparticles (CROSBI ID 702452)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Kalčec, Nikolina ; Frkanec, Ruža ; Vinković Vrček, Ivana
engleski
Impact of serum albumin on binding ability of dopamine and L-dopa on differently coated gold nanoparticles
Introduction Efficient and safe treatment of patients suffering Parkinson’s disease faces many challenges such as poor blood brain barrier penetration and decreased response to therapy with time. Nano-drug delivery systems may overcome these problems and can be designed to penetrate through the blood-brain barrier and interact with the central nervous system without causing any unwanted side effects. However, their efficiency largely depend on their stability in biological fluids like human plasma. This study evaluated impact of serum albumin on ability of novel gold nanoparticles (AuNPs) to be used as delivery system for dopamine or L-dopa and evaluated on their binding abilities. For this purpose, AuNPs functionalized with polyethylene glycol (PEG) and adamantane derivatives were prepared. Binding affinity of dopamine and L-dopa for these AuNPs was evaluated in the presence or absence of serum albumin using steady-state fluorescence spectroscopy. Results Differences in binding constants were observed for L-dopa/dopamine on differently functionalized AuNPs. The observed quenching mechanism was static in nature for all novel AuNPs. The presence of serum albumin interfered with L-dopa/dopamine binding on AuNPs. Conclusions Binding constants depend not only on the different functionalization of AuNPs, but also on the presence of serum protein.
albumin, L-dopa, dopamine, gold nanoparticles
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Podaci o prilogu
118-118.
2021.
objavljeno
Podaci o matičnoj publikaciji
NanoTox 2021 Virtual Conference Book of Abstracts
Edinburgh:
Podaci o skupu
10th International Conference on Nanotoxicology (NanoTox 2021)
poster
20.04.2021-22.04.2021
Edinburgh, Ujedinjeno Kraljevstvo