engleski

Construction of AC/DC magnetic syringe device for stimulated drug release, injection and ejection of nanocarriers and testing cytotoxicity in vitro

Iron nanoparticles are used as a targeted drug delivery system. The nanocarrier itself can be genotoxic, trigger oxidative stress or cell death. Therefore, we developed an AC/DC magnetic syringe for injecting, stimulating drug release and safe removing of the nanocarrier. Alongside we optimized the method for nanoparticles’ drug release kinetics and testing cytotoxicity in vitro. • This paper presents detailed instructions for construction of AC/DC magnetic syringe device for stimulated drug release, injection and ejection of magnetic nanoparticles ; nanoparticles preparation ; adsorbing methylene blue on nanoparticles ; determination of drug release kinetics from nanocarriers on the example of methylene blue • Gomori´s Prussian blue reaction for differentiated SH-SY5Y human neuroblastoma cell line ; MTT viability assay optimized for differentiated SH-SY5Y human neuroblastoma cell line and antioxidant enzymes activities assay and lipid peroxidation methods are optimized for cell analyses cell cultivation for nanoparticles cytotoxicity testing in vitro. • Those protocols are the first step toward further testing the effect of nanoparticles in vivo, on brain tissue.

metal nanoparticles ; drug delivery systems ; cell viability ; oxidative stress ; lipid peroxidation

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