engleski

The effect of 7-day high-salt diet on serum antioxidant enzymes protein concentrations: does antioxidant vitamins supplementation matter?

Objective: It is well documented that high salt (HS) diet causes increased oxidative stress. Present study aimed to test the effect of 7-day HS diet on serum protein concentrations of Cu/Zn superoxide dismutase (Cu/Zn SOD), glutathione peroxidase 1 (GPx1) and catalase, and to examine whether the oral supplementation of non-specific ROS scavengers (vitamin C and vitamin E) during 7-day HS loading will change serum protein concentrations of these antioxidant enzymes in otherwise healthy normotensive individuals. Design and Method: 40 young healthy individuals (20 men and 20 women, age ranged 18-24) were assigned to a 7-day low-salt diet (<3.5 g salt/day) followed by a 7-day HS diet (~14 g salt/day) without (HS group) or with the daily supplementation of vitamins C (1000 mg) and E (800 IU) (HS+C+E group). Blood pressure (BP) and heart rate (HR) were measured at each study visit. Serum protein concentrations of Cu/Zn SOD, GPx1 and catalase were measured by commercially available ELISA kits before and after diet protocols. Results: BP and HR were not significantly different in both, HS and HS+C+E groups compared to LS diet. Changes in calculated daily salt intake from 24h sodium excretion confirmed that participants conformed to the diet protocols. Serum protein concentration of Cu/Zn SOD and GPx1 were not significantly changed following both HS or HS+C+E diet compared to measurement following LS diet. 7- day HS diet significantly decreased serum catalase concentration in HS group compared to LS diet (catalase pg/ml LS 347 ± 495 vs. HS 261 ± 462, P<0.05), while catalase concentration was similar after LS and following 7 days of HS+C+E diet (catalase pg/ml LS 249 ± 375 vs. HS+C+E 186 ± 349, P>0.05). Conclusions: The results of this study suggest that disturbing the activity of antioxidative defense itself (decreased serum catalase concentration) could potentially contribute to the altered oxidative status in healthy individuals on a HS diet, which could be prevented by intake of antioxidant vitamins C and E during HS diet.

high salt diet ; oxidative stress ; vitamin C ; vitamin E ; antioxidative enzymes

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