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The Antimicrobial Effect of Calasept, Superlux Calcium Hydroxide Liner and Gutta-percha with Calcium Hydroxide

Microorganisms are the main cause of disease in root canals and periapically. Facultative bacteria and fungi have been identified in persistent endodontic infection. The objective of this study was to determine in vitro the efficacy of: Calasept (Speiko, Darmstadt, Germany), Superlux calcium hydroxide liner (Dental Material Gesellschaft, Hamburg, Germany) and gutta-percha with calcium hydroxide (Roeko, Langenau, Germany) in direct contact with microorganisms. The microorganisms used were: Enterococcus faecalis (ATCC 29212) and Candida albicans (NCTC 3123). An overnight mixed broth culture of microorganisms (108 CFU/ml) was prepared. Paper points and gutta- percha points were immersed in suspension for 5 min. The paper points were then covered with Calasept and Superlux calcium hydroxide liner. At intervals of 0 ; 6 ; 12 ; 24 ; 48 and 72 h at 37°C, aerobically. The antimicrobial effect of Calasept and Superlux calcium hydroxide liner apparently occured after 6h on Enterococcus faecalis and 12 h on Candida albicans. With gutta-percha, this effect did not occur for either Enterococcus faecalis or Candida albicans after 72 h.

Candida albicans ; Enterococcus faecalis ; Calasept ; Superlux calcium hidroxide liner ; gutta-percha with calcium hydroxide

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