engleski

Optimization of ultrasound-assisted solid-liquid extraction for the phenolic acids recovery from barley husk

The ultrasound-assisted solid-liquid extraction of phenolic acids from barley husk was optimized using response surface methodology(RSM). A Box- Behnken design was used to investigate the effects of extraction temperature (40-80 °C), solvent concentration(ethanol/water50-90%, v/v), time (10- 30 min) and ultrasonicpower (40-80%), as well as their interaction on the extractability of phenolic acids (3, 4-dihydroxybenzoic, p- hydroxybenzoic acid, p-hydroxyphenylacetic, vanillic, caffeic, syringic, p-coumaric, ferulic, ellagic, o-coumaric acids).Barley husk obtained from winter barley variety“Favorit”(BcInstitute d.d. Zagreb, Croatia)was used.The extraction was performed using the ultrasonic bath at sweep mode and ultrasonic frequency of 37 kHz. Individual phenolic acids from barley husk extracts were quantified by UHPLC and the sumof their content was employed in the optimizationprocedure.RSM analysis showed that solvent concentration had a significant effecton phenolic acids recovery, while the effectsof other factors and their interactions were not significant.The optimal extraction conditions were found to be 80 °C, 50%, v/vethanol/water, 40% ultrasonic power and 10 min. At these conditions, the predicted value ofphenolic acids yield was 134 μg/gdb.

ultrasound-assisted extraction ; phenolic acids ; barley husk ; response surface methodology

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