engleski

Production of xylanase during cultivation of Trametes versicolor on sugar beet pulp

Sugar beet pulp (SBP) is a by-product of the sugar beet (Beta vulgaris L.) processing in sucrose production and is primarily used as animal feed. Around 20 million tons of SBP is produced in Europe each year. The dry basis of SBP is mainly composed of polysaccharides (cellulose, hemicellulose and pectin), along with protein, and small amounts of lignin, fat and ash. Hemicellulose is consisted of xylan, a heteropolysaccharide substituted with monosaccharides (L-arabinose, D-galactose, D- mannoses) and organic acids (acetic, ferulic and glucuronic acid), interwoven together with glycosidic and ester bonds. Xylanase (endo-1, 4-b- D-xylanase, xylanohydrolase, EC 3.2.1.8) is an important xylan degrading enzyme involved in the random cleavage of xylan to produce xylose, xylobiose and xylo-oligosaccharides. In this work, SBP was used as a substrate for Trametes versicolor cultivation in solid-state conditions during 8 days for the purpose of xylanase production. Xylanase activity of 318 U/g DM was reached after 8 days of cultivation under following initial process conditions: T = 27 °C, pH 4.5, m (SBP) = 25 g, m (added water) = 60 cm3, five mycelial plugs (Ø 1 cm).

sugar beet pulp ; xylanase ; Trametes versicolor

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