engleski

Enhancement of phenolic acids content of grape pomace as a consequence of treatment with Ganoderma resinaceum

The genus Ganoderma belongs to the Basydiomycota division, family Ganodermataceae. Fungi of Ganoderma genus are known for their healing properties and prevention of various diseases in humans. They have been used in traditional Asian medicine since ancient times. Ganoderma resinaceum is white rot fungus, which can degrade plant cell wall by performing selective delignification or simultaneous rot. The aim of this work was to enhance the extractability of phenolic acids from grape pomace (GP) after solid-state treatment with G. resinaceum for 15 days. Extraction of phenolic acids from GP was performed with 50% ethanol. Ten phenolic acids were analyzed by UHPLC. Biological treatment had a positive effect on production of seven phenolic acids. Initial content of phenolic acids such as gallic acid (GA), protocatechuic acid (PHA), syringic acid (SA), o-coumaric acid (o-CuA), p-hydroxybenzoic acid (p-HBA), p-coumaric acid (p-CuA) and vanillic acid (VA) in the untreated GP were 0.200 mg/gdb, 0.134 mg/gdb, 0.079 mg/gdb, 0.007 mg/gdb, 0.005 mg/gdb, 0.005 mg/gdb, 0.034 mg/gdb, respectively. After biological treatment, the content was higher up to 1.59-, 2.03-, 1.25-, 1.15-, 1.25-, 2.09- and 1.21- fold for GA, PHA, SA, o-CuA, p-HBA, p-CuA and VA, respectively.

grape pomace ; phenolic acids ; solid-state fermentation ; Ganoderma resinaceum

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