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Facile electrochemical determination and characterization of bioflavonoid hesperidin using disposable pencil graphite electrode – Application in pharmaceutical analysis

This study is focused on simple voltammetric determination of hesperidin by using electroactivated PGE in model systems and in real samples in order to evaluate the adequacy of the developed method in pharmaceutical analysis. Electroactivation of the electrode was performed in various potentials ranging from -1.4 V to 2.0 V in the phosphate buffer pH = 7.0. The electrochemical properties of hesperidin in model systems were studied with cyclic (CV) and differential pulse voltammetry (DPV). Identification and quantification of hesperidin in pharmaceutical formulation was obtained with DPV. The results have indicated that hesperidin is irreversibly oxidized, with the most pronounced oxidation at pH = 5.0. Oxidation process was under mixed diffusion and adsorption control and included the equal number of protons and electrons. The peak current and the hesperidin concentration showed linear dependence in the concentration range from 5x10-7 mol dm-3 to 1x10-5 mol dm-3. Estimated limit of detection (LOD) was 5x10-7 mol dm-3. The method was applied for determination of hesperidin in pharmaceutical formulation (containing active substance, hesperidin and excipients) and good correlation was obtained between experimentally obtained and expected hesperidin concentration (R2 = 0.9347), which showed potential application of developed method for hesperidin detection in pharmaceuticals.

hesperidin ; electrochemistry ; disposable pencil graphite electrode

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