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Pregled bibliografske jedinice broj: 1117347

CpG islands in MyD88 and ASC/PYCARD/TMS1 promoter regions are differentially methylated in head and neck squamous cell carcinoma and primary lung squamous cell carcinoma


Šutić, Maja; Baranašić, Jurica; Kovač Bilić, Lana; Bilić, Mario; Jakovčević, Antonija; Brčić, Luka; Seiwerth, Sven; Jakopović, Marko; Samaržija, Miroslav; Zechner, Ulrich; Knežević, Jelena
CpG islands in MyD88 and ASC/PYCARD/TMS1 promoter regions are differentially methylated in head and neck squamous cell carcinoma and primary lung squamous cell carcinoma // Diagnostic pathology, 16 (2021), 17, 5 doi:10.1186/s13000-021-01078-3 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1117347 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
CpG islands in MyD88 and ASC/PYCARD/TMS1 promoter regions are differentially methylated in head and neck squamous cell carcinoma and primary lung squamous cell carcinoma

Autori
Šutić, Maja ; Baranašić, Jurica ; Kovač Bilić, Lana ; Bilić, Mario ; Jakovčević, Antonija ; Brčić, Luka ; Seiwerth, Sven ; Jakopović, Marko ; Samaržija, Miroslav ; Zechner, Ulrich ; Knežević, Jelena

Izvornik
Diagnostic pathology (1746-1596) 16 (2021); 17, 5

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
CpG ; diagnostic biomarker ; HNSCC ; lung ; methylation ; second primary tumor

Sažetak
Background: Patients with head and neck squamous cell carcinoma (HNSCC) can develop lung squamous cell carcinoma (LuSCC), which could be the second primary tumor or HNSCC metastasis. Morphologically it is difficult to distinguish metastatic HNSCC from a second primary tumor which presents a significant diagnostic challenge. Differentiation of those two malignancies is important because the recommended treatments for metastatic HNSCC and primary LuSCC differ significantly. We investigated if the quantification of the promotor methylation status in HNSCC and LuSCC differs. Methods: Primary HNSCC (N = 36) and LuSCC (N = 17) were included in this study. Methylation status in the ASC/TMS1/PYCARD (apoptosis- associated speck-like protein containing a caspase recruitment domain ; 8 CpG sites) and MyD88 (Myeloid differentiation primary response protein 88 ; 10 CpG sites) promoters was analyzed. Bisulfite converted DNA, isolated from tumor tissue was quantified using pyrosequencing. Results of pyrosequencing analysis were expressed as a percentage for each tested CpG site. Receiver-operating characteristic (ROC) curve analysis was used for the evaluation of the diagnostic properties of selected biomarkers. Results: CpG sites located in the promoters of ASC/TMS1/PYCARD_CpG8 (- 65 upstream) and MyD88_CpG4 (- 278 upstream) are significantly hypermethylated in the HNSCC when compared with LuSCC (p ≤ 0.0001). By performing ROC curve analysis we showed that corresponding areas under the curve (AUC) were 85-95%, indicating that selected CpG sites are useful for a distinction between primary LuSCC and primary HNSCC. Conclusions: Results of the present study indicate that there is a significant difference in the methylation status of tested genes between primary HNSCC and LuSCC. However, to prove this approach as a useful tool for distinguishing second primary LuSCC from HNSCC metastasis, it would be necessary to include a larger number of samples, and most importantly, metastatic samples.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti



POVEZANOST RADA


Projekti:
HRZZ-IP-2016-06-1441 - Genetički i epigenetički biomarkeri urođene imunosti u KOPB-u i karcinomu pluća (LungInflaCare) (Knežević, Jelena, HRZZ - 2016-06) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb,
Klinički bolnički centar Zagreb,
Fakultet za dentalnu medicinu i zdravstvo, Osijek

Poveznice na cjeloviti tekst rada:

doi diagnosticpathology.biomedcentral.com fulir.irb.hr

Citiraj ovu publikaciju:

Šutić, Maja; Baranašić, Jurica; Kovač Bilić, Lana; Bilić, Mario; Jakovčević, Antonija; Brčić, Luka; Seiwerth, Sven; Jakopović, Marko; Samaržija, Miroslav; Zechner, Ulrich; Knežević, Jelena
CpG islands in MyD88 and ASC/PYCARD/TMS1 promoter regions are differentially methylated in head and neck squamous cell carcinoma and primary lung squamous cell carcinoma // Diagnostic pathology, 16 (2021), 17, 5 doi:10.1186/s13000-021-01078-3 (međunarodna recenzija, članak, znanstveni)
Šutić, M., Baranašić, J., Kovač Bilić, L., Bilić, M., Jakovčević, A., Brčić, L., Seiwerth, S., Jakopović, M., Samaržija, M., Zechner, U. & Knežević, J. (2021) CpG islands in MyD88 and ASC/PYCARD/TMS1 promoter regions are differentially methylated in head and neck squamous cell carcinoma and primary lung squamous cell carcinoma. Diagnostic pathology, 16, 17, 5 doi:10.1186/s13000-021-01078-3.
@article{article, author = {\v{S}uti\'{c}, Maja and Barana\v{s}i\'{c}, Jurica and Kova\v{c} Bili\'{c}, Lana and Bili\'{c}, Mario and Jakov\v{c}evi\'{c}, Antonija and Br\v{c}i\'{c}, Luka and Seiwerth, Sven and Jakopovi\'{c}, Marko and Samar\v{z}ija, Miroslav and Zechner, Ulrich and Kne\v{z}evi\'{c}, Jelena}, year = {2021}, pages = {5}, DOI = {10.1186/s13000-021-01078-3}, chapter = {17}, keywords = {CpG, diagnostic biomarker, HNSCC, lung, methylation, second primary tumor}, journal = {Diagnostic pathology}, doi = {10.1186/s13000-021-01078-3}, volume = {16}, issn = {1746-1596}, title = {CpG islands in MyD88 and ASC/PYCARD/TMS1 promoter regions are differentially methylated in head and neck squamous cell carcinoma and primary lung squamous cell carcinoma}, keyword = {CpG, diagnostic biomarker, HNSCC, lung, methylation, second primary tumor}, chapternumber = {17} }
@article{article, author = {\v{S}uti\'{c}, Maja and Barana\v{s}i\'{c}, Jurica and Kova\v{c} Bili\'{c}, Lana and Bili\'{c}, Mario and Jakov\v{c}evi\'{c}, Antonija and Br\v{c}i\'{c}, Luka and Seiwerth, Sven and Jakopovi\'{c}, Marko and Samar\v{z}ija, Miroslav and Zechner, Ulrich and Kne\v{z}evi\'{c}, Jelena}, year = {2021}, pages = {5}, DOI = {10.1186/s13000-021-01078-3}, chapter = {17}, keywords = {CpG, diagnostic biomarker, HNSCC, lung, methylation, second primary tumor}, journal = {Diagnostic pathology}, doi = {10.1186/s13000-021-01078-3}, volume = {16}, issn = {1746-1596}, title = {CpG islands in MyD88 and ASC/PYCARD/TMS1 promoter regions are differentially methylated in head and neck squamous cell carcinoma and primary lung squamous cell carcinoma}, keyword = {CpG, diagnostic biomarker, HNSCC, lung, methylation, second primary tumor}, chapternumber = {17} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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