Wavelength dependent photochemistry of BODIPY-phenols and their applications in fluorescent labeling of proteins (CROSBI ID 292333)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Zlatić, Katarina ; Cindrić, Matej ; Antol, Ivana ; Uzelac, Lidija ; Mihaljević, Branka ; Kralj, Marijeta ; Basarić, Nikola
engleski
Wavelength dependent photochemistry of BODIPY-phenols and their applications in fluorescent labeling of proteins
A series of BODIPY dyes were synthesized, that were at the 3, or 3 and 5 positions, substituted by photochemically reactive quinone methide (QM) precursor moieties. Fluorescence properties of the molecules were investigated and we demonstrated that the molecules undergo wavelength dependent photochemistry. Photodeamination to deliver QMs takes place only upon excitation to higher excited singlet states, showing unusual anti-Kasha photochemical reactivity. The findings were corroborated by TD-DFT computations. Laser flash photolysis experiments could not reveal QMs due to low efficiency of their formation, but enabled detection of the phenoxyl radicals. The applicability of the molecules for fluorescent labeling of bovine serum albumin as a model protein upon photoexcitation at 350 nm was demonstrated.
Anti-Kasha photochemistry ; BODIPY ; Quinone methides ; Protein labelling
Rad je na naslovnici Organic & Biomolecular Chemistry, Volume 19, Number 2214, June 2021 https://mail.irb.hr/?_task=mail&_uid=50601&_mbox=INBOX&_action=get&_part=2
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Podaci o izdanju
19 (22)
2021.
4891-4903
objavljeno
1477-0520
1477-0539
10.1039/D1OB00278C
Povezanost rada
Biologija, Kemija, Temeljne medicinske znanosti