engleski

The influence of albumin on biomimetic formation of calcium phosphates / TiO2 nanowires composites

Composite materials based on calcium phosphates (CaPs) and TiO2 nanomaterials have recently attracted attention as new and innovative implant materials for bone regeneration.1 In addition, they can be prepared by the biomimetic method, considered to be a green synthesis route. In vivo, implant performance is affected by surface adsorption of soluble proteins from blood and interstitial fluids. Among them, albumin is the most abundant. However, its role in the formation of CaPs on titanium implants is still not clarified. In order to fill this void, the influence of albumin on biomimetic formation CaPs on TiO2 nanowires (TiNW) has been investigated. The TiNWs were prepared using a hydrothermal method. CaPs/TiNW composites were prepared by precipitating CaPs in the presence of TiNW. Precipitation systems were prepared by fast mixing of equal volumes of CaCl2 and Na2HPO4 solutions in the double-walled glass vessel. TiNW and/or BSA were added in Na2HPO4 solution. The progress of the reaction was followed by monitoring the changes of pH in the precipitation systems. Formed precipitates were filtered after 60 minutes of reaction time and characterized by Fourier – transform infrared spectroscopy, Raman spectroscopy, X-ray powder diffraction, scanning electron microscopy and transmission electron microscopy. It was shown that TiNWs act as promotors while BSA acts as an inhibitor of CaP formation. None of the additives influenced the composition and the morphology of the formed precipitate. The obtained results point to a biomimetic preparation route of multifunctional CaP based biominerals.

albumine ; biomimetic synthesis, ; calcium phosphates ; TiO2 nanowires

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