A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterization in different cell types (CROSBI ID 291669)
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Podaci o odgovornosti
Miserey-Lenkei S, Trajkovic K, D'Ambrosio JM, Patel AJ, Čopič A, Mathur P, Schauer K, Goud B, Albanèse V, Gautier R, Subra M, Kovacs D, Barelli H, Antonny B.
engleski
A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterization in different cell types
Comprehensive libraries of plasmids for SARS-CoV- 2 proteins with various tags (e.g. Strep, HA, Turbo) are now available. They enable the identification of numerous potential protein- protein interactions between the SARS-CoV-2 virus and host proteins. To facilitate further cellular investigations, notably by imaging techniques, we present here a large library of SARS CoV-2 protein constructs fused with green and red fluorescent proteins and their initial characterization in various human cell lines including lung epithelial cell models (A549, BEAS-2B), as well as in budding yeast. The localization of a few SARS-CoV-2 proteins matches their proposed interactions with host proteins. These include the localization of Nsp13 to the centrosome, Orf3a to late endosomes, and Orf9b to mitochondria.
SARS-CoV-2
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