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izvor podataka: crosbi

Macrodomain protein from Streptomyces coelicolor - SCO6735 reverses T-linked DNA ADP-ribosylation (CROSBI ID 700262)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Hloušek-Kasun, Andrea ; Mikolčević, Petra ; Jankevicius, Gytis ; Tromans-Coia, Callum ; Sabljić, Igor ; Bertoša, Branimir ; Ahel, Ivan ; Mikoč, Andreja Macrodomain protein from Streptomyces coelicolor - SCO6735 reverses T-linked DNA ADP-ribosylation // PARP 2019 / Curtin, Nikola ; Corda, Daniela ; Yelamos, Jose et al. (ur.). Budimpešta, 2019

Podaci o odgovornosti

Hloušek-Kasun, Andrea ; Mikolčević, Petra ; Jankevicius, Gytis ; Tromans-Coia, Callum ; Sabljić, Igor ; Bertoša, Branimir ; Ahel, Ivan ; Mikoč, Andreja

engleski

Macrodomain protein from Streptomyces coelicolor - SCO6735 reverses T-linked DNA ADP-ribosylation

Diverse proteins that constitute ADP- ribosylation metabolic cycle have been found in bacteria from the genus Streptomyces. We focused on the macrodomain family of ADP- ribosyl hydrolases that encompass key players in the recognition, interpretation, and turnover of ADP-ribose signaling. In Streptomyces, ADP-ribosylation is involved in the regulation of morphological differentiation and antibiotic production. Our results on ADP- ribosyl hydrolase from S. coelicolor - SCO6735 showed that disruption of SCO6735 increases production of antibiotic actinorhodin. We structurally and biochemically characterized SCO6735 protein. The crystal structure of SCO6735 revealed highly conserved three- layered α-β-α sandwich macrodomain fold with a deep cleft that represents a putative ligand- binding site. Currently, we are employing different molecular modelling methods in order to pinpoint catalytic mechanism of SCO6735 protein. Experiments in vitro showed that SCO6735 is active on both protein and DNA modified substrates ; it efficiently reverses protein ADP-ribosylation on glutamate residues and DNA ADP-ribosylation on thymidines that have been modified by DarT toxin. SCO6735 activity in vivo has been shown in E. coli ; when SCO6735 and DarT are co-expressed, SCO6735 can rescue E. coli from the toxic effect of DarT.

ADP-ribosylation, ADP-ribosyl hydrolase, DNA ADP-ribosylation, Macrodomain, Streptomyces coelicolor

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Podaci o prilogu

28

2019.

objavljeno

Podaci o matičnoj publikaciji

PARP 2019

Curtin, Nikola ; Corda, Daniela ; Yelamos, Jose ; Bai, Peter

Budimpešta:

Podaci o skupu

PARP 2019

poster

20.05.2019-23.05.2019

Budimpešta, Mađarska

Povezanost rada

Biologija, Biotehnologija, Kemija