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Pregled bibliografske jedinice broj: 1112994

Protocol for enzymatic desialylation of native apo-transferrin


Friganović, Tomislav; Borko, Valentina; Šeba, Tino; Kerep, Robert; Biruš, Ivan; Weitner, Tin
Protocol for enzymatic desialylation of native apo-transferrin, 2020. doi:10.5281/zenodo.4002005 (ostalo).


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Naslov
Protocol for enzymatic desialylation of native apo-transferrin

Autori
Friganović, Tomislav ; Borko, Valentina ; Šeba, Tino ; Kerep, Robert ; Biruš, Ivan ; Weitner, Tin

Vrsta, podvrsta
Ostale vrste radova, ostalo

Godina
2020

Ključne riječi
transferrin, desialylation, neuraminidase, enzyme

Sažetak
This protocol describes the method used for desialylation of native apo-transferrin. Desialylation is done by incubation of immobilized neuraminidase enzyme in the buffered solution of native apo-transferrin for 48 hours. After incubation, the desialylated sample is collected, washed out using a suitable buffer and concentrated by centrifugal filtration. The percentage recovery of the obtained desialylated transferrin is calculated from the absorbance at 280 nm.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija



POVEZANOST RADA


Projekti:
UIP-2017-05-9537 - Glikozilacija serumskog transferina kao faktor u mehanizmu prijenosa željeza (GlyMech) (Weitner, Tin, HRZZ - 2017-05) ( POIROT)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb

Profili:

Avatar Url Valentina Borko (autor)

Avatar Url Tino Šeba (autor)

Avatar Url Robert Kerep (autor)

Avatar Url Tin Weitner (autor)

Avatar Url Tomislav Friganović (autor)

Avatar Url Ivan Biruš (autor)

Poveznice na cjeloviti tekst rada:

doi zenodo.org

Citiraj ovu publikaciju:

Friganović, Tomislav; Borko, Valentina; Šeba, Tino; Kerep, Robert; Biruš, Ivan; Weitner, Tin
Protocol for enzymatic desialylation of native apo-transferrin, 2020. doi:10.5281/zenodo.4002005 (ostalo).
Friganović, T., Borko, V., Šeba, T., Kerep, R., Biruš, I. & Weitner, T. (2020) Protocol for enzymatic desialylation of native apo-transferrin.. Ostalo doi:10.5281/zenodo.4002005.
@unknown{unknown, author = {Friganovi\'{c}, Tomislav and Borko, Valentina and \v{S}eba, Tino and Kerep, Robert and Biru\v{s}, Ivan and Weitner, Tin}, year = {2020}, DOI = {10.5281/zenodo.4002005}, keywords = {transferrin, desialylation, neuraminidase, enzyme}, doi = {10.5281/zenodo.4002005}, title = {Protocol for enzymatic desialylation of native apo-transferrin}, keyword = {transferrin, desialylation, neuraminidase, enzyme} }
@unknown{unknown, author = {Friganovi\'{c}, Tomislav and Borko, Valentina and \v{S}eba, Tino and Kerep, Robert and Biru\v{s}, Ivan and Weitner, Tin}, year = {2020}, DOI = {10.5281/zenodo.4002005}, keywords = {transferrin, desialylation, neuraminidase, enzyme}, doi = {10.5281/zenodo.4002005}, title = {Protocol for enzymatic desialylation of native apo-transferrin}, keyword = {transferrin, desialylation, neuraminidase, enzyme} }

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