engleski

Protocol for enzymatic desialylation of native apo-transferrin

This protocol describes the method used for desialylation of native apo-transferrin. Desialylation is done by incubation of immobilized neuraminidase enzyme in the buffered solution of native apo-transferrin for 48 hours. After incubation, the desialylated sample is collected, washed out using a suitable buffer and concentrated by centrifugal filtration. The percentage recovery of the obtained desialylated transferrin is calculated from the absorbance at 280 nm.

transferrin, desialylation, neuraminidase, enzyme

nije evidentirano