engleski

High voltage electrical discharges in extractions of bioactives from Oregano leaves (Origanum vulgare L.): process control and impact on antioxidative properties of extract

According to UN Agenda 2030. there is great concern of future trends in environmental protection, food production, health, water etc. Extractions are commonly considered as to be energy consuming and solvent consuming processes. High voltage electrical discharge- plasma (HVED) is one of new promising green techniques and “green” solvent can be selected using computer approach by COSMO-RS. Plasma generator IMP-SSPG-1200 was used for extractions (Impel group, Zagreb, Croatia). Optimization of processing extraction parameters (argon and nitrogen gases for generation of plasma, voltage – 15kV, 20kV and/or 25kV), frequency (100Hz), pulse duration (400 ms) and treatment times (3 and 9 min) was done using software STATGRAPHICS Centurion XV StatPoint (STATPOINT TECHNOLOGIES, INC., Warrenton, Virginia, USA). Oregano prepared by pharmacopeia (1g per 50mL of solvents (water or water/ethanol (75/25 v/v ; 50/50 v/v) was treated by HVED. Extraction process was controlled by analytical methods (determination of amount (yield) of total phenolic compounds, antioxidant properties by EPR (electronic paramagnetic resonance) method, DPPH ; FRAP ; near infrared spectroscopy (NIR), FTIR and differential scanning calorimetry (DSC), and investigate changes during and after extraction process. Process follows 6 principles of “green” extraction through development an equipment for generating HVED plasma ; effectiveness of extraction of bioactive compounds from Oregano and increased yield by optimization of the process parameters. Results showed increased yield of obtained extracts, increased antioxidant activity of extracts, and no changes in DSC and FTIR.

High voltage electrical discharges ; Oregano ; Electronic paramagnetic resonance ; Near infrared spectroscopy

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