engleski

Influence of conventional and innovative extraction techniques on bioactive properties of ground ivy (Glechoma hederacea L.)

Glechoma hederacea L., known as ground ivy, is a perennial plant commonly grown in Europe, Asia and America. Although it has been used for generations in folk medicine [1], the linkage between its bioactive content and health benefits still requires scientific ground. The aim of this study was to investigate the bioactive potential of Glechoma hederacea L. extracts prepared by conventional (infusion, decoction and maceration) and innovative (high hydrostatic pressure extraction, ultrasound assisted extraction and subcritical water extraction) methods of extraction using water as a solvent. The obtained extracts were spectrophotometrically evaluated for total phenolic content, antioxidant capacity (DPPH and ABTS assays) and the content of hydroxycinnamic acid derivatives. Individual phenolics profile was determined using HPLC- DAD. Of all extraction techniques, the extract prepared by decoction (100 °C, 20 min) resulted in the highest total phenolic content (37.39 mg GAE g-1), antioxidant capacity (162.53 and 177.24 mmol Trolox g-1) and hydroxycinnamic acid derivatives content (15.90 mg caffeic acid g-1), with rosmarinic acid as predominant (2.00 mg g- 1). Among the applied innovative methods, the highest values for evaluated parameters were obtained for extract prepared by high hydrostatic pressure (200 MPa, 20 min), having caffeic acid as dominant (0.48 mg g-1).

Glechoma hederacea L. ; innovative methods of extraction ; bioactive content

nije evidentirano