Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 1112152

A cascade reaction for the synthesis of D- fagomine precursor revisited: kinetic insight and understanding of the system


Sudar, Martina; Česnik, Morana; Clapés, Pere; Pohl, Martina; Vasić-Rački, Đurđa; Findrik Blažević, Zvjezdana
A cascade reaction for the synthesis of D- fagomine precursor revisited: kinetic insight and understanding of the system // New Biotechnology, 63 (2021), 19-28 doi:10.1016/j.nbt.2021.02.004 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1112152 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
A cascade reaction for the synthesis of D- fagomine precursor revisited: kinetic insight and understanding of the system

Autori
Sudar, Martina ; Česnik, Morana ; Clapés, Pere ; Pohl, Martina ; Vasić-Rački, Đurđa ; Findrik Blažević, Zvjezdana

Izvornik
New Biotechnology (1871-6784) 63 (2021); 19-28

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cascade reaction, D-fagomine precursor, mathematical model, reaction optimization

Sažetak
The synthesis of aldol adduct (3S, 4R)-6- [(benzyloxycarbonyl)amino]-5, 6-dideoxyhex-2- ulose, a precursor of an interesting dietary supplement iminosugar D-fagomine, was studied in a cascade reaction with three enzymes starting from Cbz-N-3-aminopropanol. This system was studied previously using a statistical optimization method which enabled a 79% yield of the aldol adduct with a 10% yield of the undesired amino acid by-product. Here, a kinetic model of the cascade, including enzyme operational stability decay rate and the undesired overoxidation of the intermediate product, was developed. The validated model was instrumental in the optimization of the cascade reaction in the batch reactor. Simulations were carried out to determine the variables with the most significant impact on substrate conversion and product yield. As a result, process conditions were found that provided the aldol adduct in 92% yield with only 0.7% yield of the amino acid in one-pot one-step reaction. Additionally, compared to previous work, this improved process outcome was achieved at lower concentrations of two enzymes used in the reaction. With this study the advantages are demonstrated of a modelling approach in developing complex biocatalytical processes. Mathematical models enable better understanding of the interactions of variables in the investigated system, reduce cost, experimental efforts in the lab and time necessary to obtain results since the simulations are carried out in silico.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija



POVEZANOST RADA


Ustanove:
Fakultet kemijskog inženjerstva i tehnologije, Zagreb

doi

Citiraj ovu publikaciju

Sudar, Martina; Česnik, Morana; Clapés, Pere; Pohl, Martina; Vasić-Rački, Đurđa; Findrik Blažević, Zvjezdana
A cascade reaction for the synthesis of D- fagomine precursor revisited: kinetic insight and understanding of the system // New Biotechnology, 63 (2021), 19-28 doi:10.1016/j.nbt.2021.02.004 (međunarodna recenzija, članak, znanstveni)
Sudar, M., Česnik, M., Clapés, P., Pohl, M., Vasić-Rački, Đ. & Findrik Blažević, Z. (2021) A cascade reaction for the synthesis of D- fagomine precursor revisited: kinetic insight and understanding of the system. New Biotechnology, 63, 19-28 doi:10.1016/j.nbt.2021.02.004.
@article{article, year = {2021}, pages = {19-28}, DOI = {10.1016/j.nbt.2021.02.004}, keywords = {cascade reaction, D-fagomine precursor, mathematical model, reaction optimization}, journal = {New Biotechnology}, doi = {10.1016/j.nbt.2021.02.004}, volume = {63}, issn = {1871-6784}, title = {A cascade reaction for the synthesis of D- fagomine precursor revisited: kinetic insight and understanding of the system}, keyword = {cascade reaction, D-fagomine precursor, mathematical model, reaction optimization} }
@article{article, year = {2021}, pages = {19-28}, DOI = {10.1016/j.nbt.2021.02.004}, keywords = {cascade reaction, D-fagomine precursor, mathematical model, reaction optimization}, journal = {New Biotechnology}, doi = {10.1016/j.nbt.2021.02.004}, volume = {63}, issn = {1871-6784}, title = {A cascade reaction for the synthesis of D- fagomine precursor revisited: kinetic insight and understanding of the system}, keyword = {cascade reaction, D-fagomine precursor, mathematical model, reaction optimization} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font