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Pregled bibliografske jedinice broj: 1111897

Determination of Adenylate Nucleotides in Amphipod Gammarus fossarum by Ion-Pair Reverse Phase Liquid Chromatography: Possibilities of Positive Pressure Micro-Solid Phase Extraction


Redžović, Zuzana; Erk, Marijana; Svetličić, Ema; Dončević, Lucija; Gottstein, Sanja; Hozić, Amela; Cindrić, Mario
Determination of Adenylate Nucleotides in Amphipod Gammarus fossarum by Ion-Pair Reverse Phase Liquid Chromatography: Possibilities of Positive Pressure Micro-Solid Phase Extraction // Separations, 8 (2021), 8020020, 11 doi:10.3390/separations8020020 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1111897 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Determination of Adenylate Nucleotides in Amphipod Gammarus fossarum by Ion-Pair Reverse Phase Liquid Chromatography: Possibilities of Positive Pressure Micro-Solid Phase Extraction

Autori
Redžović, Zuzana ; Erk, Marijana ; Svetličić, Ema ; Dončević, Lucija ; Gottstein, Sanja ; Hozić, Amela ; Cindrić, Mario

Izvornik
Separations (2297-8739) 8 (2021); 8020020, 11

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
adenylate energy charge ; freshwater amphipods ; ecosystem monitoring ; adenylate extraction ; ecological friendly solutions

Sažetak
Adenine nucleotides—adenosine monophosphate, diphosphate, and triphosphate—are of utmost importance to all living organisms, where they play a critical role in the energy metabolism and are tied to allosteric regulation in various regulatory enzymes. Adenylate energy charge represents the precise relationship between the concentrations of adenosine monophosphate, diphosphate, and triphosphate and indicates the amount of metabolic energy available to an organism. The experimental conditions of adenylate extraction in freshwater amphipod Gammarus fossarum are reported here for the first time and are crucial for the qualitative and quantitative determination of adenylate nucleotides using efficient and sensitive ion-pair reverse phase LC. It was shown that amphipod calcified exoskeleton impeded the neutralization of homogenate. The highest adenylate yield was obtained by homogenization in perchloric acid and subsequent addition of potassium hydroxide and phosphate buffer to achieve a pH around 11. This method enables separation and accurate detection of adenylates. Our study provides new insight into the complexity of adenylate extraction and quantification that is crucial for the application of adenylate energy charge as a confident physiological measure of environmental stress and as a health index of G. fossarum.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Interdisciplinarne prirodne znanosti



POVEZANOST RADA


Projekti:
EK-EFRR-KK.01.1.1.07.0023 - Qua/Qua Protein: Kvantitativna i kvalitativna analiza proteina za potrebe biomedicine i biotehnološke industrije (Qua/Qua Protein) (Cindrić, Mario, EK - KK.01.1.1.07) ( POIROT)
HRZZ-IP-2014-09-4255 - Akumulacija, unutarstanično mapiranje i učinci metala u tragovima u akvatičkih organizama (AQUAMAPMET) (Erk, Marijana, HRZZ - 2014-09) ( POIROT)

Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb,
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Citiraj ovu publikaciju:

Redžović, Zuzana; Erk, Marijana; Svetličić, Ema; Dončević, Lucija; Gottstein, Sanja; Hozić, Amela; Cindrić, Mario
Determination of Adenylate Nucleotides in Amphipod Gammarus fossarum by Ion-Pair Reverse Phase Liquid Chromatography: Possibilities of Positive Pressure Micro-Solid Phase Extraction // Separations, 8 (2021), 8020020, 11 doi:10.3390/separations8020020 (međunarodna recenzija, članak, znanstveni)
Redžović, Z., Erk, M., Svetličić, E., Dončević, L., Gottstein, S., Hozić, A. & Cindrić, M. (2021) Determination of Adenylate Nucleotides in Amphipod Gammarus fossarum by Ion-Pair Reverse Phase Liquid Chromatography: Possibilities of Positive Pressure Micro-Solid Phase Extraction. Separations, 8, 8020020, 11 doi:10.3390/separations8020020.
@article{article, author = {Red\v{z}ovi\'{c}, Zuzana and Erk, Marijana and Svetli\v{c}i\'{c}, Ema and Don\v{c}evi\'{c}, Lucija and Gottstein, Sanja and Hozi\'{c}, Amela and Cindri\'{c}, Mario}, year = {2021}, pages = {11}, DOI = {10.3390/separations8020020}, chapter = {8020020}, keywords = {adenylate energy charge, freshwater amphipods, ecosystem monitoring, adenylate extraction, ecological friendly solutions}, journal = {Separations}, doi = {10.3390/separations8020020}, volume = {8}, issn = {2297-8739}, title = {Determination of Adenylate Nucleotides in Amphipod Gammarus fossarum by Ion-Pair Reverse Phase Liquid Chromatography: Possibilities of Positive Pressure Micro-Solid Phase Extraction}, keyword = {adenylate energy charge, freshwater amphipods, ecosystem monitoring, adenylate extraction, ecological friendly solutions}, chapternumber = {8020020} }
@article{article, author = {Red\v{z}ovi\'{c}, Zuzana and Erk, Marijana and Svetli\v{c}i\'{c}, Ema and Don\v{c}evi\'{c}, Lucija and Gottstein, Sanja and Hozi\'{c}, Amela and Cindri\'{c}, Mario}, year = {2021}, pages = {11}, DOI = {10.3390/separations8020020}, chapter = {8020020}, keywords = {adenylate energy charge, freshwater amphipods, ecosystem monitoring, adenylate extraction, ecological friendly solutions}, journal = {Separations}, doi = {10.3390/separations8020020}, volume = {8}, issn = {2297-8739}, title = {Determination of Adenylate Nucleotides in Amphipod Gammarus fossarum by Ion-Pair Reverse Phase Liquid Chromatography: Possibilities of Positive Pressure Micro-Solid Phase Extraction}, keyword = {adenylate energy charge, freshwater amphipods, ecosystem monitoring, adenylate extraction, ecological friendly solutions}, chapternumber = {8020020} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Uključenost u ostale bibliografske baze podataka::


  • CAPlus / SciFinder (American Chemical Society)


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