Immunogenicity of Newcastle disease virus strain ZG1999HDS applied oculonasally or by means of nebulisation to day-old chicks (CROSBI ID 291036)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Cvetić, Željko ; Nedeljković, Gordana ; Jergović, Mladen ; Bendelja, Krešo ; Mazija, Hrvoje ; Gottstein, Željko
engleski
Immunogenicity of Newcastle disease virus strain ZG1999HDS applied oculonasally or by means of nebulisation to day-old chicks
Newcastle disease (ND) is one of the classic viral infections of poultry which resists all the efforts of eradication. Newcastle disease virus (NDV) strain ZG1999HDS was isolated during the outbreak in 1, 999 at a broiler farm in Croatia. Previous trials in chickens confirmed it to be a lentogenic pathotype and immunogenic by stimulating humoral and cell mediated immunity. Further characterization by deduced amino acid sequence at the cleavage site of fusion protein confirmed its lentogenic nature, and in vitro tests its oncolytic capacity. Owing to its immunogenicity, strain ZG1999HDS is considered for vaccine development. In this study, 1-day-old chicks were vaccinated using strain ZG1999HDS oculonasally or by nebulization. Strain ZG1999HDS induced humoral immune response in both immunized groups The cell-mediated immune response occurred earlier in the group immunized by nebulization, as shown by a higher frequency rate of T and B lymphocytes, and significantly higher expression of IFN-α in respiratory organs and IFN-γ expression in the spleen. Viral genomic RNA was not detected in investigated organs. Thus, NDV strain ZG1999HDS is immunogenic when administered by means of nebulization or oculonasally without any adverse effects and is therefore suitable for further research and vaccine development. Further research is needed regarding its tropism.
Newcastle disease ; cell-mediated immunity ; flow cytometry ; interferon-α ; interferon- γ
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Podaci o izdanju
Trošak objave rada u otvorenom pristupu
Povezanost rada
Biologija, Interdisciplinarne biotehničke znanosti, Veterinarska medicina