hrvatski

Učinak levamisola na histološki i imunohistokemijski nalaz u tankom crijevu odbite prasadi cijepljene protiv kolibaciloze

nije evidentirano

levamisol; odbita prasad; cijepljenje; tanka crijeva

In the present work we aimed to test the hypothesis that levamisole exerts its adjuvant activity in the vaccinated pigs by priming T lymphocytes in the gut mucosa , e.g.ileal Peyer's patch (IPP), or jejunal lamina propria (JLP).Experimeltal group of weaned pigs was primed with levamisole in immunostimulatory dose of 2, 5, mg/kg given daily , in 3 consecutive days , and controls received saline according to the same schedule.Both group were orally vaccinated with the vaccinal E.coli strain on day 0 and challenged with the virulent E.coli strain 7 days later.All pigs were sacrificed on post-challenge day 6.Expresion of CD3 antigens by IPP and LP T cells was tested using flow cytometry and immunohistochemistry.By the quantitative phenotypic analysis of isolated lymphocytes, CD3+ IPP but not LP T cells icreased in experimental vs control pigs, implying that levamisole exerts its adjuvant activity in the IPP by augmenting of cells that participate in cell-mediated immunity.The results of the quantitative phenotypic analysis of isolated cells were confirmed by immunohistochemical in situ staining.Moreover, immunohistocemical analysis of the gut mucosa revealed the presence of numerous CD