engleski

Sucrose cleaving enzymes and differentiation relationships in sugarbeet cell lines

Sugars like glucose and sucrose are regulators of gene expression (sugar-sensing), aside from being substrate for metabolism. On each step during differentiation, te availability, concentration and utilisation of sugars are tightly controled and monitored by the cell. Previous results showed using NMR that sugarbeet cell lines have different concentrations of intracellular sugars (sucrose, glucose and fructose) regarding to their differentiation status. In order to test potential sugar-sensing mechanism, activities of sugar cleaving enyzmes: invertase, sucrose synthase and sucrose phosphate synthase were measured using different cell lines cultivated on gelfield medium containing different carbohydrates. Protein expression was analysed by western blot to confirm enzymatic activities. Results showed that the different cell lines had distinct patterns of enzymatic activities when grown on the same sugar and that activities changed when different exogenous sugars (glucose or fructose) were added to culture medium. Further work will be to test this regulation at transcriptional level.

sugarbeel cell lines

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